Institute of Biotechnology , RWTH Aachen University , Worringer Weg 3 , D-52074 Aachen , Germany.
DWI - Leibniz-Institute for Interactive Materials , Forckenbeckstrasse 50 , D-52074 Aachen , Germany.
Bioconjug Chem. 2019 Mar 20;30(3):714-720. doi: 10.1021/acs.bioconjchem.8b00874. Epub 2019 Jan 29.
Adhesion promoting peptides have been reported to enable efficient enzyme immobilization on various material surfaces. Here we report the first immobilization of a synthetic Grubbs-Hoveyda (GH) type catalyst on two different materials (silica and polypropylene). To this end, the GH catalyst was coupled to an engineered (F16C) variant of the adhesion promoting peptide LCI through thiol-maleimide "click" reaction. Immobilization was performed in an oriented manner through the adhesion promoting peptide by simple incubation with the materials in water and subsequent washing with water and tetrahydrofuran. The immobilized GH catalyst was probed in ring-opening metathesis polymerization of a norbornene derivative to alter the surface properties in a layer-by-layer fashion.
已报道称，粘附促进肽可实现各种材料表面上酶的高效固定化。在这里，我们首次将合成的 Grubbs-Hoveyda (GH) 型催化剂固定在两种不同的材料（二氧化硅和聚丙烯）上。为此，通过巯基-马来酰亚胺“点击”反应将 GH 催化剂偶联到粘附促进肽 LCI 的工程（F16C）变体上。通过简单地将材料在水中孵育并随后用水和四氢呋喃洗涤，通过粘附促进肽以定向方式进行固定化。固定化的 GH 催化剂用于在降冰片烯衍生物的开环复分解聚合中进行探测，以按层状方式改变表面性质。
