Kamolvit Witchuda, Nilsén Vera, Zambrana Silvia, Mohanty Soumitra, Gonzales Eduardo, Östenson Claes-Göran, Brauner Annelie
Department of Microbiology, Tumor and Cell Biology, Division of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, 17176 Stockholm, Sweden.
Department of Molecular Medicine and Surgery, Karolinska Institutet and Karolinska University Hospital, 17176 Stockholm, Sweden.
Evid Based Complement Alternat Med. 2018 Dec 16;2018:1098015. doi: 10.1155/2018/1098015. eCollection 2018.
is a South American herb with edible beans, known to reduce serum glucose levels in diabetic patients. Furthermore, contains phytochemicals known to decrease bacterial load. Based on the increased urinary tract infections experienced among patients with diabetes, we investigated the effect of on bladder epithelial cells in the protection of infection during normal and high glucose concentrations. We did not observe any direct antibacterial effect by extract. Instead we observed an influence on the host cells, with indirect impact on bacteria and their possibility of causing infection. extract decreased adhesion to bladder epithelial cells of uropathogenic bacteria, including drug-resistant strains. Moreover, uroplakin1a, involved in adhesion, was downregulated while the antimicrobial peptide RNase 7 was upregulated in treated cells irrespectively of glucose concentration. This supports an early effect fighting bacteria. Additionally, prevented bacterial biofilm formation, which is used by bacteria to evade the immune system and antibiotics. In summary, protects against bacterial infection in uroepithelial cells by preventing adhesion through alteration of the cell surface, increasing antimicrobial peptide expression, and reducing biofilm formation. Together, this promotes bacterial clearance, suggesting that as extract or as a dietary item can contribute to the prevention of urinary tract infections, which is of importance in an era of increasing antibiotic resistance.
是一种南美洲的草本植物，其豆可食用，已知能降低糖尿病患者的血清葡萄糖水平。此外，它含有已知能降低细菌载量的植物化学物质。基于糖尿病患者中尿路感染增加的情况，我们研究了其在正常和高葡萄糖浓度下对膀胱上皮细胞在预防感染方面的作用。我们未观察到其提取物有任何直接抗菌作用。相反，我们观察到其对宿主细胞有影响，对细菌及其引起感染的可能性有间接影响。其提取物降低了包括耐药菌株在内的尿路致病性细菌对膀胱上皮细胞的黏附。此外，无论葡萄糖浓度如何，参与黏附的uroplakin1a在其处理的细胞中下调，而抗菌肽RNase 7上调。这支持了早期对抗细菌的作用。此外，它还能防止细菌生物膜形成，而细菌利用生物膜来逃避免疫系统和抗生素。总之，它通过改变细胞表面来防止黏附、增加抗菌肽表达以及减少生物膜形成，从而保护尿路上皮细胞免受细菌感染。综合起来，这促进了细菌清除，表明其作为提取物或作为饮食项目有助于预防尿路感染，这在抗生素耐药性增加的时代具有重要意义。
