Corner L A, Trajstman A C
CSIRO, Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Vet Microbiol. 1988 Oct;18(2):127-34. doi: 10.1016/0378-1135(88)90058-2.
For the primary isolation of Mycobacterium bovis from bovine lesions, 1-hexadecylpyridinium chloride (HPC) at a concentration of 0.75% was as effective as 2% NaOH in controlling the growth of contamination. The advantages of using HPC over NaOH are that it is a rapid one-step procedure not requiring neutralisation with acid, it is less toxic to M. bovis thus increasing isolation rates, and it promotes the earlier appearance of colonies.
为了从牛病变组织中初次分离牛分枝杆菌，浓度为0.75%的十六烷基氯化吡啶（HPC）在控制污染菌生长方面与2%的氢氧化钠效果相当。使用HPC相对于氢氧化钠的优势在于，它是一个快速的一步法程序，无需用酸中和，对牛分枝杆菌毒性较小，从而提高分离率，并且能促使菌落更早出现。
