Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidãde de São Paulo , São Paulo, SP , Brasil.
Braz J Microbiol. 2008 Apr;39(2):241-4. doi: 10.1590/S1517-83822008000200008. Epub 2008 Jun 1.
SIXTY SAMPLES OF TISSUE FRAGMENTS WITH LESIONS SUGGESTIVE OF TUBERCULOSIS FROM BOVINE ABATTOIRS, KEPT IN SATURATED SOLUTION OF SODIUM BORATE, WERE SUBJECTED TO FOUR TREATMENTS: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
六十份疑似结核病的牛组织样本，保存在硼酸钠饱和溶液中，经过四种处理：4%NaOH（Petroff 法）、12%H2SO4 和 1.5%HPC（1-十六烷基氯化吡啶）去污，以及生理盐水（对照）。与对照组（88%，p<0.001）、NaOH（33%，p<0.001）和 H2SO4（21.7%，p<0.002）相比，HPC 法的污染率最低（3%）。关于分离成功率，HPC 法（40%）优于对照组（3%，p<0.001）、NaOH 法（13%，p=0.001）和 H2SO4 法（1.7%，p<0.001）。这些结果表明 HPC 是 Petroff 法的替代方法。
