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[通过免疫酶分析法鉴定流感病毒的血凝素抗原]

[Identification of the hemagglutinating antigens of the influenza virus by immunoenzyme analysis].

作者信息

Isaeva E I, Rovnova Z I, Selivanov Ia M

出版信息

Vopr Virusol. 1988 Sep-Oct;33(5):538-43.

PMID:3064428
Abstract

Highly active test sera detecting the presence of virus antigen both in concentrated and purified preparations and in allantoic virus cultures directly adsorbed on the solid phase have been proposed for successful identification and detection of influenza A and B virus variants. After direct sorption of purified and concentrated virus preparations, the test sera to influenza A (H1N1, H2N2, H3N2) virus detect the virus antigen in a concentration of 8 ng/ml, test sera to influenza B virus in a concentration of 40 ng/ml. After sorption on the solid phase of allantoic virus cultures the test sera detected influenza A virus antigen in a dose of 0.25-1 agglutinating units (AU), and antigen of influenza B virus in a dose of 1-2 AU.

摘要

已提出高活性检测血清,可用于成功鉴定和检测甲型和乙型流感病毒变种,该血清能在浓缩和纯化制剂中以及直接吸附在固相上的尿囊病毒培养物中检测到病毒抗原。在对纯化和浓缩的病毒制剂进行直接吸附后,针对甲型流感病毒(H1N1、H2N2、H3N2)的检测血清能检测到浓度为8 ng/ml的病毒抗原,针对乙型流感病毒的检测血清能检测到浓度为40 ng/ml的病毒抗原。在尿囊病毒培养物吸附到固相上后,检测血清能检测到剂量为0.25 - 1个凝集单位(AU)的甲型流感病毒抗原以及剂量为1 - 2 AU的乙型流感病毒抗原。

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