Veselov S Iu, Siniakov M S, Kharitonenkov I G, Gitel'man A K, Vasiaev A I
Vopr Virusol. 1985 Nov-Dec;30(6):672-5.
A test system for the detection and quantitation of influenza virus hemagglutinin (HA) in biological specimens by solid-phase enzyme immunoassay (SPEIA) is described. The test system implies sequential adsorption on polystyrene base of anti-HA guinea pig gammaglobulins, detectable HA-containing antigen, monospecific anti-HA rabbit serum, and peroxidase antirabbit conjugate. Adsorption of the HA-containing antigen is run in the presence of a high concentration of nonionic detergent. The developed method is highly sensitive (0.3-0.5 ng in a specimen) and permits the detection and quantitation of HA in whole influenza virions, preparations of surface glycoproteins and in preparations of hemagglutinin recovered from virus particles. The analysis (after pre-sensitization of the polystyrene adsorbent) takes from 2 to 21/2 hours. The possibility of using the developed test system for control and standardization of inactivated influenza vaccines (whole-virion, split, and subunit) is discussed.
描述了一种通过固相酶免疫测定法（SPEIA）检测和定量生物标本中流感病毒血凝素（HA）的测试系统。该测试系统包括将抗HA豚鼠γ球蛋白、可检测的含HA抗原、单特异性抗HA兔血清和过氧化物酶抗兔缀合物依次吸附在聚苯乙烯基质上。含HA抗原的吸附在高浓度非离子洗涤剂存在下进行。所开发的方法高度灵敏（标本中0.3 - 0.5纳克），可检测和定量全流感病毒粒子、表面糖蛋白制剂以及从病毒颗粒中回收的血凝素制剂中的HA。分析（在聚苯乙烯吸附剂预致敏后）耗时2至2.5小时。讨论了使用所开发的测试系统对灭活流感疫苗（全病毒、裂解和亚单位）进行控制和标准化的可能性。
