Organic and Bioorganic Chemistry, Department of Chemistry, Bielefeld University, Universitätsstraße 25, D-33501 Bielefeld, Germany.
Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City-Cairo 11884, Egypt.
Z Naturforsch C J Biosci. 2019 May 27;74(5-6):131-137. doi: 10.1515/znc-2018-0145.
As a continuation of our earlier research concerning the investigation of microbial bioactive secondary metabolites from the terrestrial Penicillium sp.KH Link 1809 isolate KHMM, the fungus was re-cultivated on a large scale to explore its bioactive compounds intensively. Fifteen compounds, including seven alkaloids (1-7), one sesquiterpene (8), an acetylenic system (9), two sterols, and sphengolipid, were identified. Their structures were established on the bases of extensive one- and two-dimensional nuclear magnetic resonance and mass measurements, and by comparison with literature data. The antimicrobial activity of the fungal extract and the corresponding compounds were studied using a panel of pathogenic microorganisms, and their in vitro cytotoxicity against the human cervix carcinoma cell line (KB-3-1) was reported as well. The molecular docking of the isolated compounds showed promising affinities for the alkaloidal compounds 4-6 towards α, β tubulins.
作为我们之前研究的延续，我们对陆地青霉 KH Link 1809 菌株 KHMM 的微生物生物活性次生代谢产物进行了研究，该真菌被大规模再培养，以深入探索其生物活性化合物。鉴定了 15 种化合物，包括 7 种生物碱（1-7）、1 种倍半萜（8）、1 个炔属系统（9）、2 种甾醇和 sphengolipid。根据广泛的一维和二维核磁共振和质谱测量，并与文献数据进行比较，确定了它们的结构。使用一系列致病菌研究了真菌提取物和相应化合物的抗菌活性，并报告了它们对人宫颈癌细胞系（KB-3-1）的体外细胞毒性。分离化合物的分子对接表明，生物碱化合物 4-6 对α，β微管蛋白具有良好的亲和力。
