Opt Lett. 2019 Jan 1;44(1):1-4. doi: 10.1364/OL.44.000001.
We have developed photoacoustic microscopy (PAM) with three-dimensional (3D) micron-level spatial resolution. With multi-angle illumination, PAM images from different view angles can be simultaneously acquired for multi-view deconvolution, without the rotation of imaging targets. A side-by-side comparison of this multi-angle-illumination PAM (MAI-PAM) and conventional PAM, which share the same ultrasonic detector, was performed in phantoms and live mice. The phantom study showed that MAI-PAM achieved a high axial resolution of 3.7 μm, which was 10-fold higher than that of conventional PAM and approached the lateral resolution of 2.7 μm. Furthermore, the in vivo study demonstrated that MAI-PAM was able to image the 3D microvasculature with isotropic spatial resolution.
我们开发了具有三维（3D）微米级空间分辨率的光声显微镜（PAM）。通过多角度照明，可以同时获取来自不同视角的 PAM 图像，用于多角度反卷积，而无需旋转成像目标。在体模和活体小鼠中，对共享相同超声探测器的多角度照明 PAM（MAI-PAM）和传统 PAM 进行了并排比较。体模研究表明，MAI-PAM 实现了 3.7μm 的高轴向分辨率，是传统 PAM 的 10 倍，接近 2.7μm 的侧向分辨率。此外，体内研究表明，MAI-PAM 能够以各向同性的空间分辨率对 3D 微血管成像。
