Peterson C M, Polizzi C M
Sansum Medical Research Foundation, Santa Barbara, CA 93105.
Alcohol. 1987 Nov-Dec;4(6):477-80. doi: 10.1016/0741-8329(87)90089-9.
A fluorigenic high performance liquid chromatographic (HPLC) assay for the determination of acetaldehyde in plasma and hemoglobin-associated acetaldehyde is described. The assay is based on the reaction of acetaldehyde with two molecules of 1,3-cyclohexanedione in the presence of ammonium ion to form a fluorescent species followed by reverse phase HPLC separation of specific aldehyde derived compounds. The assay is specific; and has sensitivity in the picomole range, with intraassay precision of less that 3.5% and interassay precision of less than 15%. The total run time is less than 6 minutes on HPLC. Hemoglobin-associated acetaldehyde levels were higher than the levels found in plasma. Endogenous levels of acetaldehyde in samples obtained from teetotalers were found to be 0.43 +/- 0.04 (S.D.) microM in plasma and 74.2 +/- 16.1 nmol/g protein as hemoglobin-associated acetaldehyde. The levels of both plasma acetaldehyde and hemoglobin-associated acetaldehyde were significantly higher in patients reporting to a center for alcohol treatment than the levels encountered in teetotalers.
本文描述了一种用于测定血浆中乙醛及与血红蛋白结合的乙醛的荧光高效液相色谱（HPLC）分析法。该分析法基于乙醛在铵离子存在下与两分子1,3 - 环己二酮反应形成荧光物质，随后通过反相HPLC分离特定的醛衍生化合物。该分析法具有特异性，在皮摩尔范围内具有灵敏度，批内精密度小于3.5%，批间精密度小于15%。在HPLC上总运行时间少于6分钟。与血红蛋白结合的乙醛水平高于血浆中的水平。从戒酒者获取的样本中，血浆中乙醛的内源性水平为0.43±0.04（标准差）微摩尔，与血红蛋白结合的乙醛为74.2±16.1纳摩尔/克蛋白质。到酒精治疗中心就诊的患者血浆乙醛和与血红蛋白结合的乙醛水平均显著高于戒酒者。
