Pal Tuhin, Ghosh Samanwita, Mondal Animesh, De Kalyan Kumar
Department of Botany, Hooghly Mohsin College, Burdwan University, Chinsurah-712 101, Hooghly, West Bengal, India.
J Genet Eng Biotechnol. 2016 Jun;14(1):39-48. doi: 10.1016/j.jgeb.2016.03.003. Epub 2016 Apr 11.
Somatic chromosome study from root tip cells using the squash technique of the cytological method and seed protein profile of 5 varieties of (Lentil) through SDS-PAGE were investigated. Karyotype analysis showed gross uniformity in morphology. Somatic chromosome number 2 = 14 is constant for all the varieties. Chromosomes are mostly long to medium in length with secondary constrictions in one pair of chromosome. Primary constrictions in chromosome ranged from nearly median to nearly submedian in most of the cases. Notwithstanding the gross homogeneity, karyotype analysis revealed minute differences in details. Each lentil variety is thus characterized by its own karyotype, serving as one of the identifying criteria. The seed protein profile revealed that varieties are very close to each other with respect to similarity index that ranged from 0.594 to 0.690. With regard to seed protein banding patterns, slight polymorphism (14.285%) indicating low genetic diversity has been identified among the 5 varieties. A dendrogram indicates one variety is plesiomorphic and rest varieties are apomorphic. All the experimental varieties of lentil studied here show low genetic diversity due to their similar genetic base, indigenous genetic resources and conservative nature of the seed protein.
采用细胞学方法的压片技术对根尖细胞进行体细胞染色体研究，并通过SDS-PAGE对5个小扁豆品种的种子蛋白图谱进行了分析。核型分析表明形态上总体一致。所有品种的体细胞染色体数2n = 14是恒定的。染色体大多为长至中等长度，一对染色体上有次缢痕。在大多数情况下，染色体的主缢痕从近中部到近亚中部不等。尽管总体上具有同质性，但核型分析揭示了细节上的微小差异。因此，每个小扁豆品种都有其独特的核型特征，可作为鉴别标准之一。种子蛋白图谱显示，品种间的相似性指数在0.594至0.690之间，彼此非常接近。关于种子蛋白条带模式，在5个品种中鉴定出轻微的多态性(14.285%)，表明遗传多样性较低。聚类图表明一个品种是近祖型的，其余品种是衍生型的。由于遗传基础相似、本地遗传资源以及种子蛋白的保守性，这里研究的所有小扁豆实验品种都显示出较低的遗传多样性。
