Samynathan Ramkumar, Perisamy Suresh Kumar, Gandhi Sudhakar, Anitha J, Sanmugam Geetha, Padmanabhan Mohankumar, Velliyur Kanniappan Gopalakrishnan
Department of Biochemistry and Bioinformatics, Karpagam University, Coimbatore 641021, TN, India.
Department of Biotechnology, Anna University, BIT Campus, Tiruchirappalli, TN 620024, India.
J Genet Eng Biotechnol. 2016 Jun;14(1):69-75. doi: 10.1016/j.jgeb.2015.12.004. Epub 2016 Jan 18.
Green tea is one of the most important beverages consumed across the world and it possesses various phytotherapeutics. Polyphenol oxidase (PPO) activity, total polyphenols, catechins, amino acid content and enzymatic antioxidants are considered to be potential parameters in tea characterization. P/11/15 clone ( (L) O. Kuntze) was chosen to analyze the biochemical characterization and to analyze the gene expression pattern. The selected P/11/15 clone ( (L) O. Kuntze) possess potent Polyphenol oxidase (49.62 U/mg of protein), sufficient catechin (20.75%), Polyphenol (20.01%), Peroxidase (450.08 μM of O formed min g dry weight), Catalase (1.20 μM HO reduced min mg protein) and Super Oxide Dismutase (45.11 U/mg proteins). Flavonoid gene expression reveals ANR (1.66%) and F3H (1.02%) were up regulated in the selected P/11/15 clone. The results obtained suggest that P/11/15 clone showed adequate enzyme levels, thus an increased antioxidant activity.
绿茶是全球消费的最重要饮品之一，它具有多种植物疗法功效。多酚氧化酶（PPO）活性、总多酚、儿茶素、氨基酸含量和酶促抗氧化剂被认为是茶叶特性的潜在参数。选择P/11/15克隆株（(L) O. Kuntze）来分析其生化特性并分析基因表达模式。所选的P/11/15克隆株（(L) O. Kuntze）具有强大的多酚氧化酶（49.62 U/mg蛋白质）、充足的儿茶素（20.75%）、多酚（20.01%）、过氧化物酶（450.08 μM O每分钟形成量/克干重）、过氧化氢酶（1.20 μM H₂O₂每分钟还原量/毫克蛋白质）和超氧化物歧化酶（45.11 U/mg蛋白质）。类黄酮基因表达显示，在所选的P/11/15克隆株中，ANR（1.66%）和F3H（1.02%）上调。所得结果表明，P/11/15克隆株显示出足够的酶水平，因此抗氧化活性增强。
