Lee Si Eun, Hwang Hyun Jin, Ha Jung-Sun, Jeong Han-Seung, Kim Jeong Hee
Department of Biochemistry, College of Dentistry, Kyung Hee University, 1 Hoegi-Dong, Dongdaemoon-Ku, Seoul 130-701, South Korea.
Life Sci. 2003 May 30;73(2):167-79. doi: 10.1016/s0024-3205(03)00259-5.
The methanol extracts of nine medicinal plants traditionally used in Chinese medicine were screened for antioxidant activity versus resveratrol, which has been shown to protect cells from oxidative damage [Toxicol. Lett. 102 (1998) 5]. Most of the plant extracts used in this study inhibited the H(2)O(2)-induced apoptosis of Chinese hamster lung fibroblast (V79-4) cells. The extracts of Areca catechu var. dulcissima, Paeonia suffruticosa, Alpinia officinarum, Glycyrrhiza uralensis and Cinnamomun cassia strongly enhanced viability against H(2)O(2)-induced oxidative damage in V79-4 cells. Relatively high levels of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were detected in extracts of Areca catechu var. dulcissima, Paeonia suffruticosa and Cinnamomun cassia (IC(50) < 6.0 microg/ml). The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were dose-dependently enhanced in V79-4 cells treated with most of the plant extracts. The extracts of Areca catechu var. dulcissima showed higher antioxidant activity than resveratrol in all experiments. These results suggest that the plant extracts prevent oxidative damage in normal cells probably because of their antioxidant characteristics.
对九种传统用于中药的药用植物的甲醇提取物进行了抗氧化活性筛选,以白藜芦醇作为对照,白藜芦醇已被证明可保护细胞免受氧化损伤 [《毒理学快报》102 (1998) 5]。本研究中使用的大多数植物提取物抑制了过氧化氢诱导的中国仓鼠肺成纤维细胞(V79-4)凋亡。槟榔、牡丹、高良姜、甘草和肉桂的提取物强烈增强了V79-4细胞对过氧化氢诱导的氧化损伤的活力。在槟榔、牡丹和肉桂的提取物中检测到相对较高水平的1,1-二苯基-2-苦基肼(DPPH)自由基清除活性(半数抑制浓度<6.0微克/毫升)。用大多数植物提取物处理的V79-4细胞中,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPX)的活性呈剂量依赖性增强。在所有实验中,槟榔提取物的抗氧化活性均高于白藜芦醇。这些结果表明,植物提取物可能因其抗氧化特性而预防正常细胞中的氧化损伤。