Gunasegaran Thanes, Rathinam Xavier, Kasi Marimuthu, Sathasivam Kathiresan, Sreenivasan Sasidharan, Subramaniam Sreeramanan
Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Semeling, 08100 Bedong, Kedah Darul Aman, Malaysia.
Asian Pac J Trop Biomed. 2011 Aug;1(4):266-9. doi: 10.1016/S2221-1691(11)60040-3.
To isolate Salmonella from curry samples and to evaluate the drug sensitivity of the food-borne Salmonella and its susceptibility to specific plant extracts.
Salmonella was isolated from the curry samples by standard microbiological methods and was confirmed by biochemical tests. The antibiotic susceptibility test was conducted by disc diffusion method using commercially available antibiotics such as ampicillin, tetracycline, chloramphenicol, kanamycin, and penicillin. In addition, the susceptibility of the food-borne Salmonella was also evaluated against the aqueous extracts of Camelia sinensis (L.) Theaceae (tea leaves) and the Trachyspermum ammi (L.) Apiaceae ( ajwain or omum seeds).
Out of fifty curry samples, only seven samples were identified to have Salmonella contamination. The Salmonella isolates showed a significant drug resistance pattern except for kanamycin. The plant extracts showed a considerable antibacterial activity against the isolates, indicating the presence of antimicrobial principle which can be exploited after complete pharmacological investigations.
The present study demonstrates the occurrence of Salmonella in the curry samples, and shows significant drug resistance against most of the commercially available antibiotics, except kanamycin. Antimicrobial effect of the plant extracts against the food-bone Salmonella suggests that dietary including medicinal herbs would be one strategy to manage food borne pathogens.
从咖喱样品中分离沙门氏菌，并评估食源性沙门氏菌的药敏性及其对特定植物提取物的敏感性。
采用标准微生物学方法从咖喱样品中分离沙门氏菌，并通过生化试验进行确认。采用纸片扩散法，使用氨苄西林、四环素、氯霉素、卡那霉素和青霉素等市售抗生素进行药敏试验。此外，还评估了食源性沙门氏菌对山茶科植物茶（茶叶）和伞形科植物阿魏（阿魏籽或欧芹籽）水提取物的敏感性。
在50份咖喱样品中，仅有7份样品被鉴定出受到沙门氏菌污染。除卡那霉素外，沙门氏菌分离株呈现出显著的耐药模式。植物提取物对分离株显示出相当大的抗菌活性，表明存在抗菌成分，经过全面的药理学研究后可加以利用。
本研究证明了咖喱样品中存在沙门氏菌，且除卡那霉素外，对大多数市售抗生素呈现出显著的耐药性。植物提取物对食源性沙门氏菌的抗菌作用表明，包括药草在内的饮食可能是控制食源性病原体的一种策略。
