Moteriya Pooja, Padalia Hemali, Chanda Sumitra
Department of Biosciences (UGC-CAS), Saurashtra University, Rajkot 360 005, Gujarat, India.
J Genet Eng Biotechnol. 2017 Dec;15(2):505-513. doi: 10.1016/j.jgeb.2017.06.010. Epub 2017 Jul 13.
In this work, we report for the first time, synthesis of silver nanoparticles using leaf extract and evaluate its synergistic antibacterial efficacy and antioxidant potential. The synergistic antimicrobial activity of silver nanoparticles with commercial antibiotics against Gram-positive, Gram-negative bacteria and fungi was evaluated. The antioxidant potential of synthesized silver nanoparticles was evaluated by FRAP and ABTS radical scavenging antioxidant assays. UV-Vis spectra peak at 473 and color change from colorless to brown color confirmed the formation of AgNPs. X-ray diffraction (XRD) and selected area electron diffraction (SAED) pattern confirmed the crystalline nature of synthesized nanoparticles. Transmission electron microscopy (TEM) analysis revealed the average size of particles to be 15-20 nm. Fourier transform infrared spectroscopy (FTIR) revealed the functional groups leaf that was responsible for the reduction and capping process of nanoparticles. leaf extract synthesized AgNPs showed good synergistic antimicrobial against Gram-negative bacteria even better than some of the antibiotics used as positive control; they also showed antioxidant potential. The results suggest that the synthesized nanoparticles can be used to treat multidrug resistant microorganisms and stress related diseases and disorders.
在本研究中，我们首次报道了利用叶提取物合成银纳米颗粒，并评估其协同抗菌功效和抗氧化潜力。评估了银纳米颗粒与市售抗生素对革兰氏阳性菌、革兰氏阴性菌和真菌的协同抗菌活性。通过铁离子还原抗氧化能力（FRAP）和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐（ABTS）自由基清除抗氧化试验评估了合成银纳米颗粒的抗氧化潜力。紫外可见光谱在473处出现峰值以及颜色从无色变为棕色证实了银纳米颗粒的形成。X射线衍射（XRD）和选区电子衍射（SAED）图谱证实了合成纳米颗粒的晶体性质。透射电子显微镜（TEM）分析显示颗粒的平均尺寸为15 - 20纳米。傅里叶变换红外光谱（FTIR）揭示了叶中负责纳米颗粒还原和包覆过程的官能团。叶提取物合成的银纳米颗粒对革兰氏阴性菌显示出良好的协同抗菌作用，甚至优于用作阳性对照的一些抗生素；它们还显示出抗氧化潜力。结果表明，合成的纳米颗粒可用于治疗多重耐药微生物以及与应激相关的疾病和病症。
