Selim Manal S, Amer Shaimaa K, Mohamed Sahar S, Mounier Marwa M, Rifaat Hala M
Microbial Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt.
Microbiology Department, Faculty of Science, Ain Shams University, Egypt.
J Genet Eng Biotechnol. 2018 Jun;16(1):23-28. doi: 10.1016/j.jgeb.2017.10.014. Epub 2017 Nov 2.
Twenty streptomycete strains were isolated from marine sediment samples collected from Nabq area, Sharm El-Sheikh, Red Sea Coast, Egypt. Four of them produce exopolysaccharides (EPS) showing marked in vitro antitumor activities. Morphological and cultural characteristics of the most significant strain (No. 3) were shown. Moreover, the sequence of this strain showed similarity with . The results reveal that EPS produced by No. 3 had high cytotoxicity reaching 51.7% and 59.1% against human tumor cells of breast and colon lines respectively. A chemical analysis of EPS indicated that the composing monosaccharides were galactouronic acid, glucose, xylose, galactose, mannose, and fructose with relative ratio of 3:1:1:2:2:1 respectively, with an average molecular weight () 1.180 × 10 g/mol and of a number average molecular weight () 1.052 × 10 g/mol. Also the EPS contained uronic acid (0.5072%) and monosaccharide sulphates (21.753%).
从埃及红海海岸沙姆沙伊赫纳卜格地区采集的海洋沉积物样本中分离出20株链霉菌菌株。其中4株产生的胞外多糖（EPS）在体外显示出显著的抗肿瘤活性。展示了最具代表性的菌株（3号）的形态和培养特征。此外，该菌株的序列显示出与……相似。结果表明，3号菌株产生的EPS具有高细胞毒性，对人乳腺癌和结肠癌细胞系的细胞毒性分别达到51.7%和59.1%。EPS的化学分析表明，其组成单糖为半乳糖醛酸、葡萄糖、木糖、半乳糖、甘露糖和果糖，相对比例分别为3:1:1:2:2:1，平均分子量（）为1.180×10 g/mol，数均分子量（）为1.052×10 g/mol。此外，EPS还含有糖醛酸（0.5072%）和单糖硫酸盐（21.753%）。
