Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University of Lisbon, 2819-516 Almada, Portugal.
UCIBIO-Applied Molecular Biosciences Unit, Chemistry and Life Sciences Departments, NOVA School of Science and Technology, NOVA University of Lisbon, 2819-516 Almada, Portugal.
Mar Drugs. 2023 Jul 23;21(7):419. doi: 10.3390/md21070419.
Marine environments represent an enormous biodiversity reservoir due to their numerous different habitats, being abundant in microorganisms capable of producing biomolecules, namely exopolysaccharides (EPS), with unique physical characteristics and applications in a broad range of industrial sectors. From a total of 67 marine-derived bacteria obtained from marine sediments collected at depths of 200 to 350 m from the Estremadura Spur pockmarks field, off the coast of Continental Portugal, the strain SPUR-41 was selected to be cultivated in a bioreactor with saline culture media and glucose as a carbon source. The bacterium exhibited the capacity to produce 1.83 g/L of EPS under saline conditions. SPUR-41 EPS was a heteropolysaccharide composed of mannose (62.55% mol), glucose (9.19% mol), rhamnose (19.41% mol), glucuronic acid (4.43% mol), galactose (2.53% mol), and galacturonic acid (1.89% mol). Moreover, SPUR-41 EPS also revealed acyl groups in its composition, namely acetyl, succinyl, and pyruvyl. This study revealed the importance of research on marine environments for the discovery of bacteria that produce new value-added biopolymers for pharmaceutical and other biotechnological applications, enabling us to potentially address saline effluent pollution via a sustainable circular economy.
海洋环境由于其众多不同的栖息地而代表着巨大的生物多样性储备，其中蕴藏着大量能够产生生物分子的微生物，即胞外多糖（EPS），它们具有独特的物理特性，可广泛应用于各个工业领域。从葡萄牙大陆海岸埃斯特雷马杜拉海脊火山口场的 200 至 350 米深的海洋沉积物中获得的总共 67 株海洋来源细菌中，选择了菌株 SPUR-41 在含盐培养基和葡萄糖作为碳源的生物反应器中进行培养。该细菌在盐度条件下能够产生 1.83 g/L 的 EPS。SPUR-41 EPS 是一种由甘露糖（62.55%mol）、葡萄糖（9.19%mol）、鼠李糖（19.41%mol）、葡萄糖醛酸（4.43%mol）、半乳糖（2.53%mol）和半乳糖醛酸（1.89%mol）组成的杂多糖。此外，SPUR-41 EPS 的组成中还含有酰基，即乙酰基、琥珀酰基和丙酮酸基。本研究揭示了对海洋环境进行研究的重要性，有助于发现能够产生新的高附加值生物聚合物的细菌，从而为制药和其他生物技术应用提供新的价值，使我们能够通过可持续的循环经济潜在地解决盐水废水污染问题。
