Department of Biology, University of Naples Federico II, Naples, Italy.
Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Napoli, Italy.
Mol Reprod Dev. 2019 Oct;86(10):1357-1368. doi: 10.1002/mrd.23114. Epub 2019 Jan 22.
This study aims to assess the effects induced by 24 hr exposure to a subtoxic copper concentration on the reproductive system (gonads, spermatozoa, and protamine-like [PL] proteins) of Mytilus galloprovincialis. Inductively coupled plasma-mass spectrometry indicated accumulation of this metal in gonads, spermatozoa, and PL proteins of exposed mussels. Further, real-time polymerase chain reaction analyses showed altered expression levels of mt10 and PL proteins genes in spermatozoa and gonads, respectively, of exposed mussels. Protamine-like proteins, which represent the main basic component of sperm chromatin of this organism, showed a higher DNA binding affinity and a different DNA binding mode in exposed mussels. Moreover, an increased amount of NaCl was required for the release from sperm nuclei of PL-III, the main PL protein component. Finally, PL proteins extracted from exposed mussels promoted DNA oxidative damage in the presence of H O These results demonstrate that the tolerable copper amount could also affect the properties of PL proteins and determine the negative effects on the reproductive system of this organism. These analyses could be useful to develop quick and efficient chromatin-based genotoxicity tests for pollution biomonitoring programs.
本研究旨在评估 24 小时暴露于亚毒性铜浓度对贻贝（Mytilus galloprovincialis）生殖系统（性腺、精子和鱼精蛋白样 [PL] 蛋白）的影响。电感耦合等离子体质谱法表明，这种金属在暴露贻贝的性腺、精子和 PL 蛋白中积累。此外，实时聚合酶链反应分析显示，暴露贻贝的精子和性腺中 mt10 和 PL 蛋白基因的表达水平发生了改变。PL 蛋白是该生物精子染色质的主要碱性成分，其 DNA 结合亲和力更高，结合模式也不同。此外，暴露贻贝的精子核中 PL-III（主要的 PL 蛋白成分）的释放需要更多的 NaCl。最后，暴露贻贝中提取的 PL 蛋白在 H 2 O 2 存在下促进 DNA 氧化损伤。这些结果表明，可耐受的铜量也可能影响 PL 蛋白的性质，并对该生物的生殖系统产生负面影响。这些分析对于开发快速有效的基于染色质的遗传毒性测试以用于污染生物监测计划可能是有用的。
