Mosavat Nima, Golkar Pooran, Yousefifard Maryam, Javed Rabia
Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran.
Department of Agricultural Engineering and Technology, Payame Noor University (PNU), Tehran, Iran.
Biotechnol Appl Biochem. 2019 May;66(3):316-322. doi: 10.1002/bab.1727. Epub 2019 Feb 4.
Thymus species are aromatic plants with diverse applications in food industries and medicine. This study was conducted to evaluate the potential effect of ZnO nanoparticles (NPs) on callus proliferation and thymol and carvacrol production in three Thymus species, that is, T. vulgaris, T. daenensis, and T. kotschyanus, and Zataria multiflora. For this purpose, callus induction was performed on Murashige and Skoog (MS) medium containing different plant growth regulators (PGRs). After optimization of callus growth, the effects of different concentrations of ZnO NPs (100 and 150 mg L ) were investigated. MS containing 2 mg L of 2, 4-dichlorophenoxy acetic acid (2,4-D) and 1 mg L of kinetin (Kin) revealed significantly highest fresh weight (0.18 g) of callus in T. kotschyanus. Callus growth rate (0.079 mm day ) was found highest in T. vulgaris under similar conditions. Moreover, highest callus induction (92.50%) was achieved by T. kotschyanus in MS containing 2.5 mg L of 2,4-D. Regarding the highest content of thymol (22.8 mg L ) and carvacrol (0.68 mg L ) evaluated by high-performance liquid chromatography, best results were achieved under 150 mg L of ZnO NPs in T. kotschyanus and T. daenesis, respectively. This is simple and cost-effective method to be applied on industrial level for production of enhanced secondary metabolites content.
百里香属植物是芳香植物，在食品工业和医药领域有多种应用。本研究旨在评估氧化锌纳米颗粒（NPs）对三种百里香属植物即普通百里香、达尼百里香和科氏百里香以及伊朗牛至愈伤组织增殖和百里香酚与香芹酚产量的潜在影响。为此，在含有不同植物生长调节剂（PGRs）的Murashige和Skoog（MS）培养基上进行愈伤组织诱导。在优化愈伤组织生长后，研究了不同浓度（100和150 mg/L）的氧化锌纳米颗粒的影响。含有2 mg/L 2,4-二氯苯氧乙酸（2,4-D）和1 mg/L激动素（Kin）的MS培养基显示，科氏百里香的愈伤组织鲜重显著最高（0.18 g）。在类似条件下，普通百里香的愈伤组织生长速率（0.079 mm/天）最高。此外，科氏百里香在含有2.5 mg/L 2,4-D的MS培养基上实现了最高的愈伤组织诱导率（92.50%）。关于通过高效液相色谱法评估的百里香酚（22.8 mg/L）和香芹酚（0.68 mg/L）的最高含量，分别在科氏百里香和达尼百里香中，150 mg/L的氧化锌纳米颗粒处理下取得了最佳结果。这是一种简单且具有成本效益的方法，可应用于工业水平以提高次生代谢产物含量的生产。
