Ding Yi-Ping, Khan Inam Ullah, Li Meng-Meng, Xian Wen-Dong, Liu Lan, Zhou En-Min, Salam Nimaichand, Li Wen-Jun
1State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-Sen University, Guangzhou, 510275, PR China.
2Department of Biological Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan.
Int J Syst Evol Microbiol. 2019 Feb;69(2):434-440. doi: 10.1099/ijsem.0.003167. Epub 2019 Jan 16.
Two Gram-stain-negative and facultative anaerobic bacteria, designated strains SYSU G00088 and YIM 73032, were isolated from sediment samples collected from hot springs in Tibet, China. Based on the analyses of the 16S rRNA gene sequences, the two isolates were observed to be a member of the family Comamonadaceae, sharing highest pairwise sequence identity with type strains of Piscinibacter defluvii SH-1 (97.8 and 97.7 %, respectively). They were able to grow in the temperature range of 37-50 °C, pH 5.0-9.0 and in the presence of up to 0.5 % (w/v) NaCl. Both strains were positive for catalase and oxidase reactions. Cells of the novel isolates were short-rods and motile by means of a polar flagellum. The chemotaxonomic features of the two strains include ubiquinone 8 as the respiratory isoprenologue, diphosphatidylglycerol, phosphatidylethanolamine and phoshatidylglycerol as the known polar lipids, and C16 : 0 and C17 : 0cyclo as major fatty acids. The genomic DNA of strains SYSU G00088 and YIM 73032 had G+C contents of 71.8 and 71.9%, respectively. Based on the analyses of the phylogenetic, chemotaxonomic, morphological and genomic data, the two isolates are considered to represent a novel species of a new genus, for which the name Calidifontimicrobium sediminis gen. nov., sp. nov. is proposed. The type strain of Calidifontimicrobium sediminis is SYSU G00088 (=KCTC 52671=CGMCC 1.13597).
从中国西藏温泉采集的沉积物样本中分离出两株革兰氏阴性兼性厌氧细菌，分别命名为SYSU G00088和YIM 73032菌株。基于16S rRNA基因序列分析，这两株分离菌被鉴定为丛毛单胞菌科的成员，与Piscinibacter defluvii SH-1模式菌株的序列相似性最高（分别为97.8%和97.7%）。它们能够在37-50 °C、pH 5.0-9.0的温度范围内生长，并且在高达0.5%（w/v）NaCl的环境中也能生长。两株菌的过氧化氢酶和氧化酶反应均呈阳性。新分离菌株的细胞为短杆状，通过极生鞭毛运动。这两株菌的化学分类特征包括：呼吸性类异戊二烯醌为泛醌8，已知的极性脂类为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油，主要脂肪酸为C16 : 0和C17 : 0环丙烷脂肪酸。SYSU G00088和YIM 73032菌株的基因组DNA的G+C含量分别为71.8%和71.9%。基于系统发育、化学分类、形态学和基因组数据的分析，这两株分离菌被认为代表了一个新属的新物种，为此提出新属名为嗜热泉沉积物微菌属（Calidifontimicrobium），新种名为嗜热泉沉积物嗜热泉沉积物微菌（Calidifontimicrobium sediminis）。嗜热泉沉积物嗜热泉沉积物微菌的模式菌株为SYSU G00088（=KCTC 52671=CGMCC 1.13597）。
