Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Department of Statistics, The Chinese University of Hong Kong, Hong Kong, Special Administrative Region, China.
Brief Bioinform. 2020 Mar 23;21(2):699-708. doi: 10.1093/bib/bby128.
miRNAs represent a type of noncoding small molecule RNA. Many studies have shown that miRNAs are widely involved in the regulation of various pathways. The key to fully understanding the regulatory function of miRNAs is the determination of the pathways in which the miRNAs participate. However, the major pathway databases such as KEGG only include information regarding protein-coding genes. Here, we redesigned a pathway database (called miR+Pathway) by integrating and visualizing the 8882 human experimentally validated miRNA-target interactions (MTIs) and 150 KEGG pathways. This database is freely accessible at http://www.insect-genome.com/miR-pathway. Researchers can intuitively determine the pathways and the genes in the pathways that are regulated by miRNAs as well as the miRNAs that target the pathways. To determine the pathways in which targets of a certain miRNA or multiple miRNAs are enriched, we performed a KEGG analysis miRNAs by using the hypergeometric test. In addition, miR+Pathway provides information regarding MTIs, PubMed IDs and the experimental verification method. Users can retrieve pathways regulated by an miRNA or a gene by inputting its names.
miRNAs 是一类非编码小分子 RNA。许多研究表明，miRNAs 广泛参与各种途径的调控。充分了解 miRNAs 调控功能的关键是确定 miRNAs 参与的途径。然而，KEGG 等主要的途径数据库仅包含关于编码蛋白基因的信息。在这里，我们通过整合和可视化 8882 个人类实验验证的 miRNA-靶相互作用（MTIs）和 150 个 KEGG 途径，重新设计了一个途径数据库（称为 miR+Pathway）。该数据库可在 http://www.insect-genome.com/miR-pathway 免费访问。研究人员可以直观地确定受 miRNAs 调控的途径以及靶向途径的 miRNAs，以及受 miRNAs 调控的途径和基因。为了确定特定 miRNA 或多个 miRNAs 的靶基因富集的途径，我们使用超几何检验对 miRNAs 进行了 KEGG 分析。此外，miR+Pathway 还提供了 MTIs、PubMed ID 和实验验证方法的信息。用户可以通过输入 miRNA 或基因的名称来检索受其调控的途径。
