Yucel Bora, Coruh Atilla, Deniz Kemal
Department of Plastic Reconstructive and Aesthetic Surgery, Ministry of Health, Elmali State Hospital, Elmali/Antalya, Turkey.
Medical Faculty, Department of Plastic Surgery, Erciyes University, Kayseri, Turkey.
J Burn Care Res. 2019 Feb 20;40(2):211-219. doi: 10.1093/jbcr/irz005.
Surrounding the zone of coagulation is the zone of stasis, which is characterized as a mix of viable and nonviable cells, capillary vasoconstriction, and ischemia. Saving the zone of stasis is a major subject of the burn wound studies. If pathological changes in the zone of stasis can be minimized, conversion of burn wounds may be prevented. The aim of this study was to investigate the effects of pentoxifylline (PTX) on the zone of stasis in burns and burn wound healing. Twenty Sprague-Dawley rats were used in this study. Comb model was used to create zone of stasis in burns. Treatment group received a total of 200 mg/kg/d of PTX in two equal doses intraperitoneally whereas isotonic saline solution was given intraperitoneally to the control group. This treatment was continued until postburn day 17. Tissue samples were taken from the burn wounds on postburn days 3, 7, and 17. Fibroblastic and vascular density, inflammatory cells, re-epithelialization rates were assessed in histopathological study. Furthermore, macroscopic healing of burn areas on the right side were compared between the groups by taking pictures on postburn day 17. PTX treatment decreased inflammation of the burn wound in the early postburn period. Comparing the necrotic area between the groups, PTX apparently had lower rate of necrosis. PTX treatment increased re-epithelialization of burns wounds. Our study concluded that systemic treatment of burns by PTX enhances burn wound healing and helps salvaging the damaged but live cells in the zone of stasis by increasing the rate of epithelization, decreasing the necrotic area and preventing the deepening of the burn wound.
凝固区周围是淤滞区，其特征是存活细胞与非存活细胞混合、毛细血管收缩和局部缺血。挽救淤滞区是烧伤创面研究的一个主要课题。如果能够将淤滞区的病理变化降至最低，就可以防止烧伤创面的转变。本研究的目的是探讨己酮可可碱（PTX）对烧伤淤滞区及烧伤创面愈合的影响。本研究使用了20只Sprague-Dawley大鼠。采用梳状模型制造烧伤淤滞区。治疗组腹腔注射PTX，剂量为200 mg/kg/d，分两次等量注射，而对照组腹腔注射等渗盐溶液。这种治疗持续到烧伤后第17天。在烧伤后第3天、第7天和第17天从烧伤创面采集组织样本。在组织病理学研究中评估成纤维细胞和血管密度、炎症细胞、再上皮化率。此外，在烧伤后第17天拍照，比较两组右侧烧伤区域的宏观愈合情况。PTX治疗在烧伤后早期减轻了烧伤创面的炎症。比较两组之间的坏死面积，PTX的坏死率明显较低。PTX治疗增加了烧伤创面的再上皮化。我们的研究得出结论，PTX全身治疗烧伤可促进烧伤创面愈合，并通过提高上皮化率、减少坏死面积和防止烧伤创面加深，帮助挽救淤滞区内受损但仍存活的细胞。
