Eyuboglu Atilla Adnan, Uysal Cagri A, Ozgun Gonca, Coskun Erhan, Markal Ertas Nilgun, Haberal Mehmet
Baskent University Faculty of Medicine, Department of Plastic and Reconstructive Surgery, Ankara, Turkey.
Baskent University Faculty of Medicine, Department of Plastic and Reconstructive Surgery, Ankara, Turkey.
Burns. 2018 Mar;44(2):386-396. doi: 10.1016/j.burns.2017.08.016. Epub 2017 Oct 9.
Stasis zone is the surrounding area of the coagulation zone which is an important part determining the extent of the necrosis in burn patients. In our study we aim to salvage the stasis zone by injecting adipose derived stromal vascular fraction (ADSVF).
Thermal injury was applied on dorsum of Sprague-Dawley rats (n=20) by the "comb burn" model as described previously. When the burn injury was established on Sprague-Dawley rats (30min); rat dorsum was separated into 2 equal parts consisting of 4 burn zones (3 stasis zone) on each pair. ADSVF cells harvested from inguinal fat pads of Sprague-Dawley rats (n=5) were injected on the right side while same amount of phosphate buffered saline (PBS) injected on the left side of the same animal. One week later, average vital tissue on the statis zone was determined by macroscopy, angiography and microscopy. Vascular density, inflammatory cell density, gradient of fibrosis and epithelial thickness were determined via immunohistochemical assay.
Macroscopic stasis zone tissue viability (32±3.28%, 57±4.28%) (p<0.01), average number of vessels (10.28±1.28, 19.43±1.72) (p<0.01), capillary count (15.67±1.97, 25.35±2.15) (p<0.01) vascular density (1.55±0.38, 2.14±0.45) (p<0.01) epithelial thickness (0.014±0.009mm, 0.024±0.0011mm) were higher on ADSVF side. Fibrosis gradient (1.87±0.51, 1.50±0.43) (p<0.01) and inflammatory cell density (1.33±0.40, 1.20±0.32) (p<0.01) were higher on the PBS side.
Macroscopic and microscopic findings determined that ADSVF has a statistically significant benefit for salvaging stasis zone on acute burn injuries.
淤血区是凝血区的周边区域，是决定烧伤患者坏死范围的重要部分。在我们的研究中，我们旨在通过注射脂肪源性基质血管成分（ADSVF）来挽救淤血区。
如前所述，采用“梳状烧伤”模型对20只Sprague-Dawley大鼠背部进行热损伤。当Sprague-Dawley大鼠建立烧伤损伤后（30分钟）；将大鼠背部平均分成2部分，每部分有4个烧伤区（3个淤血区）。从5只Sprague-Dawley大鼠腹股沟脂肪垫中采集的ADSVF细胞注射到右侧，而在同一只动物的左侧注射等量的磷酸盐缓冲盐水（PBS）。1周后，通过大体观察、血管造影和显微镜检查确定淤血区的平均存活组织。通过免疫组织化学分析确定血管密度、炎性细胞密度、纤维化梯度和上皮厚度。
ADSVF侧的大体淤血区组织存活率（32±3.28%，57±4.28%）（p<0.01）、平均血管数量（10.28±1.28，19.43±1.72）（p<0.01）、毛细血管计数（15.67±1.97，25.35±2.15）（p<0.01）、血管密度（1.55±0.38，2.14±0.45）（p<0.01）、上皮厚度（0.014±0.009mm，0.024±0.0011mm）更高。PBS侧的纤维化梯度（1.87±0.51，1.50±0.43）（p<0.01）和炎性细胞密度（1.33±0.40，1.20±0.32）（p<0.01）更高。
大体和微观研究结果表明，ADSVF对挽救急性烧伤损伤的淤血区具有统计学上的显著益处。
