Department of Surgical Oncology, Sivas Cumhuriyet University Faculty of Medicine, Sivas-Turkey.
Department of Biochemistry, Sivas Cumhuriyet University Faculty of Pharmacy, Sivas-Turkey.
Ulus Travma Acil Cerrahi Derg. 2021 Jan;27(1):9-16. doi: 10.14744/tjtes.2020.25679.
In burn wound healing, zones of burn, namely zone of hyperemia, the zone of stasis, and zone of coagulation, have crucial importance. These zones have been identified based on the pathophysiology of the burn, and treatment of burn has been improved. The zone of necrosis is treated by excision and repair through grafting. Zone of stasis fully recovers in 24-48 h if the burn treatment is managed well. Otherwise, it may convert to a zone of coagulation. Hyperemia zone is a zone that recovers itself. Recovery of the zone of stasis is very critical in burn treatment. Active oxygen radicals produced due to the hypermetabolism due to burn wounds are known to speed to the process of the zone of stasis converting into the zone of coagulation. The present experimental study aims to evaluate the effects of sildenafil and N-acetylcysteine on the zone of stasis and to establish whether they had any contribution to wound healing in burns.
In the present study, 32 four months old female Wistar Albino rats with 200±20 gr body weights were used. The rats were divided into four groups as the sham group (Group 1), the intraperitoneal group (Group 2), Sildenafil group (Group 3, intraperitoneal 10 mg/kg for 10 days), N-acetylcysteine (Group 4, intraperitoneal 100 mg/kg for 10 days). Tissue samples were collected for serum and cytopathology studies of the Malondialdehyde level, glutathione peroxidase, superoxide dismutase, and catalyze enzyme activity. All the rats were sacrificed on the 10th day of the tests edema, hyperemia, epithelial degeneration, necrosis, inflammatory infiltration and fibrosis measurements were made.
When compared with the controls, both of the treatment groups had lower tissue damage scores. MDA level was lower in Group 3 and 4 compared to Group 2 and lower in Group 3 compared to Group 4. SOD, catalase and GPH-Px levels were higher in Group 3 and Group 4 compared to Group 2 and higher in Group 3 compared to Group 4.
The results of our study conducted on an experimental burn model created by rats support that Sildenafil and N-acetylcysteine have positive effects, such as decreasing oxidative stress level and increasing wound healing in burns. Further experimental studies are required on this subject.
在烧伤创面愈合中，烧伤区域（充血区、淤滞区和凝固区）具有重要意义。这些区域是基于烧伤的病理生理学确定的，并且治疗方法也得到了改进。坏死区通过切除和植皮修复。如果烧伤处理得当，淤滞区通常在 24-48 小时内完全恢复。否则，它可能会转化为凝固区。充血区是一个可以自行恢复的区域。在烧伤治疗中，淤滞区的恢复至关重要。由于烧伤创面的高代谢，产生的活性氧自由基会加速淤滞区转化为凝固区的过程。本实验研究旨在评估西地那非和 N-乙酰半胱氨酸对淤滞区的影响，并确定它们是否对烧伤创面愈合有贡献。
本研究使用 32 只体重为 200±20 克的四个月大雌性 Wistar 白化大鼠。将大鼠分为四组：假手术组（第 1 组）、腹腔注射组（第 2 组）、西地那非组（第 3 组，腹腔注射 10mg/kg，共 10 天）、N-乙酰半胱氨酸组（第 4 组，腹腔注射 100mg/kg，共 10 天）。采集组织样本，用于血清和细胞病理学研究，检测丙二醛水平、谷胱甘肽过氧化物酶、超氧化物歧化酶和催化酶活性。所有大鼠在第 10 天进行测试后处死，测量水肿、充血、上皮变性、坏死、炎症浸润和纤维化。
与对照组相比，两组治疗组的组织损伤评分均较低。第 3 组和第 4 组的 MDA 水平低于第 2 组，第 3 组的 MDA 水平低于第 4 组。第 3 组和第 4 组的 SOD、过氧化氢酶和 GPH-Px 水平高于第 2 组，第 3 组的 SOD、过氧化氢酶和 GPH-Px 水平高于第 4 组。
我们在大鼠烧伤模型上进行的实验研究结果表明，西地那非和 N-乙酰半胱氨酸具有降低氧化应激水平和促进烧伤创面愈合的积极作用。需要进一步的实验研究。
