Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH), Elche, Spain.
Institute of Marine Research (IIM), CSIC, Vigo, Spain.
PLoS One. 2019 Jan 17;14(1):e0201509. doi: 10.1371/journal.pone.0201509. eCollection 2019.
C-reactive proteins (CRPs) are among the faster acute-phase inflammation-responses proteins encoded by one gene (hcrp) in humans and seven genes (crp1-7) in zebrafish (Danio rerio) with importance in bacterial and viral infections. In this study, we described novel preferential bindings of 25-hydroxycholesterol (25HOCh) to CRP1-7 compared with other lipids and explored the antiviral effects of both 25HOCh and CRP1-7 against spring viremia carp virus (SVCV) infection in zebrafish. Both in silico and in vitro results confirmed the antiviral effect of 25HOCh and CRP1-7 interactions, thereby showing that the crosstalk between them differed among the zebrafish isoforms. The presence of oxidized cholesterols in human atherosclerotic plaques amplifies the importance that similar interactions may occur for vascular and/or neurodegenerative diseases during viral infections. In this context, the zebrafish model offers a genetic tool to further investigate these interactions.
C-反应蛋白（CRPs）是人类中由一个基因（hcrp）编码的更快的急性期炎症反应蛋白之一，在斑马鱼（Danio rerio）中有七个基因（crp1-7），在细菌和病毒感染中具有重要作用。在这项研究中，我们描述了 25-羟胆固醇（25HOCh）与 CRP1-7 的新型优先结合，与其他脂质相比，并且探索了 25HOCh 和 CRP1-7 对斑马鱼 Spring Viremia Carp 病毒（SVCV）感染的抗病毒作用。体内和体外的结果都证实了 25HOCh 和 CRP1-7 相互作用的抗病毒作用，从而表明它们之间的串扰在不同的斑马鱼同工型之间存在差异。在人类动脉粥样硬化斑块中氧化胆固醇的存在放大了在病毒感染期间，类似的相互作用可能对血管和/或神经退行性疾病发生的重要性。在这种情况下，斑马鱼模型提供了一种遗传工具，可以进一步研究这些相互作用。
