Department of Biotechnology, Alagappa University, Science Campus, Karaikudi, 630 003, Tamil Nadu, India.
Department of Biotechnology, Alagappa University, Science Campus, Karaikudi, 630 003, Tamil Nadu, India.
Food Chem Toxicol. 2019 Mar;125:322-332. doi: 10.1016/j.fct.2019.01.008. Epub 2019 Jan 14.
In humans, the occurrence of bacterial communities in the form of biofilm is considered as a major intrinsic factor accountable for a variety of stubborn infections. Staphylococcus aureus and S. epidermidis have gained considerable attention in clinical settings owing to the formation of intractable and long-lasting biofilms in medical device. The current study has been designed to explain the biofilm inhibitory efficacy of geraniol and cefotaxime combination (GCC) against S. epidermidis and methicillin-resistant S. aureus (MRSA). Biofilm biomass quantification assay was performed to evaluate the antibiofilm activity of GCC against S. epidermidis and MRSA. The minimal biofilm inhibitory concentration of GCC was found to be 100 μg/ml of geraniol and 2 μg/ml of cefotaxime. Further, microscopic analyses ascertained the devastating potential of GCC on the test pathogens' biofilm formation. Besides biofilm inhibition, GCC also suppressed the production of extracellular polymeric substance, slime and staphyloxanthin. More, GCC significantly increased the susceptibility of the test pathogens towards human blood. Further, the results of real time PCR analysis and in vivo assay using Caenorhabditis elegans unveiled the anti-biofilm potentials of GCC. Thus, the present study demonstrates the significant use of polytherapy treatment approaches to overcome the biofilm associated infections of Staphylococcus spp.
在人类中，以生物膜形式存在的细菌群落被认为是导致各种顽固感染的主要内在因素。金黄色葡萄球菌和表皮葡萄球菌由于在医疗器械中形成难以治愈和持久的生物膜，在临床环境中引起了相当大的关注。本研究旨在解释香叶醇和头孢噻肟组合（GCC）对表皮葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的生物膜抑制功效。通过生物膜生物量定量测定法来评估 GCC 对表皮葡萄球菌和 MRSA 的抗生物膜活性。发现 GCC 的最小生物膜抑制浓度为 100μg/ml 香叶醇和 2μg/ml 头孢噻肟。此外，显微镜分析确定了 GCC 对测试病原体生物膜形成的破坏性潜力。除了抑制生物膜外，GCC 还抑制了胞外聚合物、粘液和金黄色素的产生。此外，GCC 显著增加了测试病原体对人血的敏感性。此外，使用秀丽隐杆线虫进行实时 PCR 分析和体内试验的结果揭示了 GCC 的抗生物膜潜力。因此，本研究表明，联合使用多种治疗方法是克服葡萄球菌属生物膜相关感染的有效方法。
