Laboratory of Chromosome Engineering, Department of Frontier Research and Development, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu 292-0818, Japan.
Genome Structure and Function Group, Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Curr Opin Cell Biol. 2019 Jun;58:15-25. doi: 10.1016/j.ceb.2018.12.004. Epub 2019 Jan 15.
Accurate chromosome segregation is essential for cell proliferation. The centromere is a specialized chromosomal locus, on which the kinetochore structure is formed. The centromere/kinetochore is required for the equal separation of sister chromatids to daughter cells. Here, we review recent findings on centromere-specific chromatin, including its constitutive protein components, its de novo formation and maintenance mechanisms, and our progress in analyses with synthetic human artificial chromosomes (HACs).
准确的染色体分离对于细胞增殖至关重要。着丝粒是一种特化的染色体位点，其上形成着丝粒结构。着丝粒/动粒对于姐妹染色单体均等分离到子细胞中是必需的。在这里，我们回顾了最近关于着丝粒特异性染色质的发现，包括其组成蛋白成分、从头形成和维持机制，以及我们在合成人人工染色体（HAC）分析方面的进展。
