Department of Diagnostic, Clinic and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy.
Department of Medicine and Surgery, University of Parma, Parma, Italy.
Intervirology. 2018;61(4):166-173. doi: 10.1159/000494354. Epub 2019 Jan 17.
BACKGROUND/AIMS: New antivirals are needed to supplement or replace currently used drugs. The aim of this study was to evaluate the antiviral activity of synthetic peptides derived from physiological proteins.
Vero cell monolayers were infected with herpes simplex virus 1, vesicular stomatitis virus, adenovirus, and coxsackievirus B5 strains in the presence of different concentrations of the selected peptides and viral yield was determined by plaque reduction assays to evaluate the antiviral activity of the peptides. Virucidal activity was evaluated by determining the residual infectivity of viral suspensions treated for 1 h with the peptides at the same concentrations as in the viral yield assays.
Among the investigated peptides, the killer peptide proved to exert a considerable antiviral activity against herpes simplex virus, attributable to a direct effect on virus particles, while its derivative K10S showed to be effective against the four investigated virus strains only at the highest concentration tested, yet, the inhibitory effects were only partial.
Overall, initial evidence is provided on the antiviral activity of several peptides, as well as of their derivatives. Further investigation is warranted to ascertain the mechanism of action in order to develop new potential antiviral drugs.
背景/目的：需要新的抗病毒药物来补充或替代目前使用的药物。本研究旨在评估源自生理蛋白的合成肽的抗病毒活性。
在不同浓度的选定肽存在下，用单纯疱疹病毒 1、水疱性口炎病毒、腺病毒和柯萨奇病毒 B5 株感染 Vero 细胞单层，通过噬斑减少测定法测定病毒产量，以评估肽的抗病毒活性。通过确定用肽处理 1 小时的病毒悬浮液的剩余感染性来评估病毒杀灭活性，肽的浓度与病毒产量测定中相同。
在所研究的肽中，杀伤肽对单纯疱疹病毒表现出相当大的抗病毒活性，这归因于对病毒颗粒的直接作用，而其衍生物 K10S 仅在最高测试浓度下对四种研究的病毒株有效，但抑制作用仅部分有效。
总之，提供了几种肽及其衍生物的抗病毒活性的初步证据。需要进一步研究以确定作用机制，从而开发新的潜在抗病毒药物。
