Dennison D K, Meredith G M, Shillitoe E J, Caffesse R G
Division of Periodontics, University of Texas Houston Health Science Center, Dental Branch, Houston, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995 Apr;79(4):442-8. doi: 10.1016/s1079-2104(05)80124-6.
The mechanism of activity and the antiviral spectrum of Listerine antiseptic have not been examined thoroughly. We therefore tested its effect on laboratory strains of herpes simplex type 1 and type 2 (enveloped DNA viruses), influenza A virus (enveloped RNA virus), rotavirus (nonenveloped RNA virus), and adenovirus type 5 (nonenveloped DNA virus). Each virus was mixed with an equal volume of Listerine for 30 seconds to 5 minutes, and the residual infectivity of the virus was assessed. An antiviral effect was defined as greater than 95% reduction of infectivity. Exposure to Listerine for 30 seconds had an antiviral effect against herpes simplex type-1 and type-2 (96.3% and 100% reduction in infectious virus, respectively) and influenza A (100% reduction). In contrast, rotavirus-induced plaque formation was reduced by 12.2% after 30 seconds of exposure to Listerine, whereas 5 minutes of exposure to Listerine resulted in a 21.5% increase in plaque formation. Exposure of adenovirus to Listerine had a minimal effect on the cytopathocity of the virus, with a 33.4% reduction in virus levels after 5 minutes. The antiviral activity of Listerine is thus not related to the viral genome but is probably directed to the viral envelope.
利斯特林漱口水的作用机制和抗病毒谱尚未得到充分研究。因此，我们测试了其对1型和2型单纯疱疹病毒（包膜DNA病毒）、甲型流感病毒（包膜RNA病毒）、轮状病毒（无包膜RNA病毒）和5型腺病毒（无包膜DNA病毒）实验室菌株的影响。将每种病毒与等量的利斯特林漱口水混合30秒至5分钟，然后评估病毒的残余感染力。抗病毒效果定义为感染力降低超过95%。暴露于利斯特林漱口水30秒对1型和2型单纯疱疹病毒有抗病毒作用（感染性病毒分别减少96.3%和100%）以及对甲型流感病毒（减少100%）。相比之下，暴露于利斯特林漱口水30秒后轮状病毒诱导的蚀斑形成减少了12.2%，而暴露5分钟则导致蚀斑形成增加了21.5%。腺病毒暴露于利斯特林漱口水对病毒的细胞病变效应影响最小，5分钟后病毒水平降低了33.4%。因此，利斯特林漱口水的抗病毒活性与病毒基因组无关，可能作用于病毒包膜。
