Tian Linglin, Li Yuan, Chang Ruqi, Zhang Peng, Zhang Jian, Huo Lijuan
Department of Gastroenterology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
Oncol Lett. 2019 Jan;17(1):175-182. doi: 10.3892/ol.2018.9567. Epub 2018 Oct 11.
Colorectal cancer caused by inflammatory bowel disease is referred as colitis-associated cancer (CAC). The mechanism underling CAC is not fully understood. In the present study, the role of interleukin-9 (IL-9) in CAC was examined. The current study included 12 colorectal tissue specimens and matched adjacent tissues from CAC. The expression of IL-9 protein was examined using immunohistochemical staining. The expression of IL-9 in cancer tissues was markedly higher compared with that in adjacent tissues. Furthermore, IL-9 gene overexpression lentiviral vectors were constructed to overexpress IL-9 in RKO and Caco-2 cell lines. The role of IL-9 in cell proliferation was investigated using a Cell Counting Kit-8 assay, and MYC proto-oncogene bHLH transcription factor () and expression levels were detected by reverse transcription-quantitative polymerase chain reaction. Notably, IL-9 overexpression promoted the proliferation of colonic epithelial cells by upregulating of the expression of and . In conclusion, the present results suggested that IL-9 may exhibit an essential role in the pathogenesis of CAC, and IL-9 promotes the proliferation of colonic epithelial RKO and Caco2 cells, partially via the upregulation of and expression.
炎症性肠病引起的结直肠癌被称为结肠炎相关癌（CAC）。CAC的潜在机制尚未完全阐明。在本研究中，检测了白细胞介素-9（IL-9）在CAC中的作用。本研究纳入了12例CAC患者的结直肠组织标本及其配对的相邻组织。采用免疫组织化学染色检测IL-9蛋白的表达。结果显示，癌组织中IL-9的表达明显高于相邻组织。此外，构建了IL-9基因过表达慢病毒载体，以在RKO和Caco-2细胞系中过表达IL-9。使用细胞计数试剂盒-8检测法研究IL-9在细胞增殖中的作用，并通过逆转录-定量聚合酶链反应检测MYC原癌基因bHLH转录因子（）和的表达水平。值得注意的是，IL-9过表达通过上调和的表达促进结肠上皮细胞的增殖。总之，本研究结果提示IL-9可能在CAC的发病机制中发挥重要作用，并且IL-9部分通过上调和的表达促进结肠上皮RKO和Caco2细胞的增殖。
