Zhuang Lu, Guo Jia, Yao Yao, Li Zhaoshen
Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
Department of Cancer Biology, Mayo Clinic Florida, Jacksonville, FL 32224, USA.
Oncol Lett. 2019 Jan;17(1):843-848. doi: 10.3892/ol.2018.9689. Epub 2018 Nov 12.
Previous studies have demonstrated that microRNAs (miRs) serve important roles in the progression of human cancer types, including pancreatic cancer (PC), a highly lethal malignancy. In the past few decades, several miRs have been identified to be associated with the overall survival of patients with PC and have been demonstrated to be potential therapeutic targets. However, to the best of our knowledge, the association between miR-205 expression and the progression of PC has rarely been investigated. In the current study, low miR-205 expression was revealed in PC tumor tissues and indicated poor prognosis in patients with PC. In addition, miR-205 overexpression reduced and miR-205 depletion enhanced PC cell proliferation and migration . Using bioinformatics, a luciferase reporter assay and western blot analyses, the current study identified that runt-related transcription factor 2 (RUNX2) was a target of miR-205 in PC and overexpression of miR-205 suppressed the expression of RUNX2. Notably, overexpression of RUNX2 partially reversed the inhibitory effect of miR-205 on PC cell proliferation and migration . Therefore, the results of the present study revealed that miR-205 functions as a tumor suppressor in PC by targeting RUNX2.
先前的研究表明，微小RNA（miR）在包括胰腺癌（PC）这种高致死性恶性肿瘤在内的人类癌症类型进展中发挥重要作用。在过去几十年里，已鉴定出几种miR与PC患者的总生存期相关，并已证明其为潜在的治疗靶点。然而，据我们所知，miR-205表达与PC进展之间的关联很少被研究。在当前研究中，PC肿瘤组织中miR-205表达较低，且这表明PC患者预后较差。此外，miR-205过表达减少了PC细胞增殖和迁移，而miR-205缺失则增强了PC细胞增殖和迁移。通过生物信息学、荧光素酶报告基因检测和蛋白质印迹分析，当前研究确定了 runt相关转录因子2（RUNX2）是PC中miR-205的一个靶点，且miR-205过表达抑制了RUNX2的表达。值得注意的是，RUNX2过表达部分逆转了miR-205对PC细胞增殖和迁移的抑制作用。因此，本研究结果表明，miR-205通过靶向RUNX2在PC中发挥肿瘤抑制作用。
