Zhou Xiaoqiu, Li Guoyong, Wang Dongguan, Sun Xiyin, Li Xingong
Department of Pathology, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China.
Department of Stomatology, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China.
Oncol Lett. 2019 Jan;17(1):927-932. doi: 10.3892/ol.2018.9679. Epub 2018 Nov 8.
The expression levels of seven types of cytokeratin (CK) in different kinds of skin adnexal tumors were evaluated. One hundred and thirty-two patients with different kinds of skin adnexal tumors admitted and treated in the Department of Dermatology of Dongying People's Hospital from May 2013 to May 2015 were selected and underwent tissue section staining. Another 20 cases of normal skin were enrolled as the control group. The expression levels of the seven types of CK in different kinds of skin appendages were observed and recorded. The expression levels of the seven types of CK in the 132 cases of skin adnexal tumor tissues were different. CK10 was mainly expressed in squamous cell carcinoma, but it was not expressed in basal cell carcinoma. CK19 was expressed in basal cell carcinoma, but its expression was not detected in squamous cell carcinoma. As the degree of differentiation was increased in the epidermis, hair follicle and sebaceous gland, the expressed molecular weight of CK was augmented gradually. The expression levels of five types of CK (namely, CK8, CK10, CK14, CK18 and CK19) could be measured in the squamous cell carcinoma and basal cell carcinoma. Statistical analysis revealed that there were statistically significant differences in the expression levels of these five types of CK in the two cell carcinomas (P<0.05). Five types of CK, i.e., CK7, CK8, CK17, CK18 and CK19, had markedly different expression levels in hair follicle tumor and sweat gland tumor, which were statistically significant (P<0.05). The expression levels of a group of CKs detected by virtue of semi-quantitative analysis via immunohistochemistry can be regarded as one of the important indexes for clinical diagnosis of skin adnexal tumors.
评估了七种细胞角蛋白（CK）在不同类型皮肤附属器肿瘤中的表达水平。选取2013年5月至2015年5月在东营市人民医院皮肤科收治的132例不同类型皮肤附属器肿瘤患者，进行组织切片染色。另选取20例正常皮肤作为对照组。观察并记录七种CK在不同皮肤附属器中的表达水平。132例皮肤附属器肿瘤组织中七种CK的表达水平各不相同。CK10主要表达于鳞状细胞癌，而在基底细胞癌中未表达。CK19表达于基底细胞癌，但在鳞状细胞癌中未检测到其表达。随着表皮、毛囊和皮脂腺分化程度的增加，CK表达的分子量逐渐增大。在鳞状细胞癌和基底细胞癌中可检测到五种CK（即CK8、CK10、CK14、CK18和CK19）的表达水平。统计学分析显示，这五种CK在两种细胞癌中的表达水平存在统计学差异（P<0.05）。五种CK，即CK7、CK8、CK17、CK18和CK19，在毛囊肿瘤和汗腺肿瘤中的表达水平有显著差异，具有统计学意义（P<0.05）。通过免疫组织化学半定量分析检测的一组CK的表达水平可作为皮肤附属器肿瘤临床诊断的重要指标之一。
