Liu Chen-Jian, Zhang Yuan-Lian, Shang Yun, Wu Bian, Yang En, Luo Yi-Yong, Li Xiao-Ran
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China.
Department of General Surgery, First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China.
Oncol Lett. 2019 Jan;17(1):1115-1127. doi: 10.3892/ol.2018.9714. Epub 2018 Nov 15.
Intestinal bacteria are symbiotic microbiota within the human gut and are implicated in the occurrence and development of colorectal cancer (CRC). The current study investigated the changes in bacterial composition prior to and following surgery, as well as the differences in the bacterial community structure between cancer tissue and adjacent normal tissue. The diversity of the bacterial community and the composition of the bacteria were assessed. In addition, phylogenetic analysis and principle component analysis (PCA) were performed. The results revealed that cancer tissue and adjacent normal tissue exhibited similar bacterial compositions. However, a significant difference was identified in the composition of intestinal bacteria in stool samples collected from patients following surgery compared with stool samples collected prior to surgery. Each patient had their own unique intestinal bacterial community, likely due to a number of factors, including diet, genetic factors and health status. In addition, phylogenetic trees revealed that the most abundant operational taxonomic unit, 0001, was associated with in all samples. Finally, PCA suggested that the bacterial community structure in all patient stools was similar following surgery. The current study provides information regarding the diversity of the intestinal bacterial community of patients with CRC and provides a basis for postoperative intestinal assessments.
肠道细菌是人类肠道内的共生微生物群，与结直肠癌（CRC）的发生和发展有关。本研究调查了手术前后细菌组成的变化，以及癌组织与相邻正常组织之间细菌群落结构的差异。评估了细菌群落的多样性和细菌组成。此外，进行了系统发育分析和主成分分析（PCA）。结果显示，癌组织和相邻正常组织表现出相似的细菌组成。然而，与手术前采集的粪便样本相比，手术后采集的患者粪便样本中的肠道细菌组成存在显著差异。每个患者都有自己独特的肠道细菌群落，这可能是由于多种因素造成的，包括饮食、遗传因素和健康状况。此外，系统发育树显示，在所有样本中，最丰富的可操作分类单元0001与 有关。最后，主成分分析表明，所有患者术后粪便中的细菌群落结构相似。本研究提供了有关CRC患者肠道细菌群落多样性的信息，并为术后肠道评估提供了依据。
