Agilent Technologies (Shanghai) Co. Ltd., Shanghai, 200131, China.
Environmental and Radiation Health Sciences Directorate, Health Canada, 50 Colombine Driveway, PL 0800C, Ottawa, ON, K1A 0K9, Canada.
Environ Sci Pollut Res Int. 2019 Mar;26(8):7453-7462. doi: 10.1007/s11356-018-04073-2. Epub 2019 Jan 17.
A method for the analysis of short-chain and medium-chain chlorinated paraffins (SCCPs and MCCPs) in house dust was developed. The method is based on sonication extraction, sample cleanup by solid phase extraction (SPE), and separation and detection by gas chromatography coupled with mass spectrometry (GC/MS) operated in electron capture negative ion (ECNI) chemical ionization mode. The method is sensitive, with method detection limits (MDLs) down to 0.22 μg/g for SCCPs and 0.55 μg/g for MCCPs. The overall recoveries of the method were 104 (± 11)% and 108 (± 16)% for SCCPs and MCCPs, respectively. The method was successfully applied to the analysis of SCCPs and MCCPs in NIST standard reference material (SRM 2585, organic contaminants in house dust) and a subset of house dust samples collected under the Canadian House Dust Study (CHDS). Average concentrations of SCCPs and MCCPs in SRM 2585 (n = 12 replicates) were 7.58 (± 0.43) μg/g for SCCPs and 16.4 (± 2.1) μg/g for MCCPs, respectively. A comparison was made between CP concentrations in paired dust samples collected using two different methods from the same homes: fresh or "active" dust (FD) collected by technicians and a sample taken from the household vacuum cleaner (HD). Spearman rank analysis showed a significant positive correlation (p < 0.01) between FD and HD samples for both MCCPs and SCCPs. CPs were detected in every house dust sample (n = 48 HD samples), with median (range) concentrations of 6.2 (4.0 - 57) μg/g and 19 (5.9-901) μg/g for SCCPs and MCCPs, respectively. Widely scattered CP levels and 100% detection frequency in this preliminary set of 48 HD samples suggest a wide variability in Canadian household exposures to CPs.
建立了一种用于分析房屋灰尘中短链和中链氯化石蜡（SCCPs 和 MCCPs）的方法。该方法基于超声提取、固相萃取（SPE）样品净化，以及通过气相色谱-质谱联用（GC/MS）在电子捕获负离子（ECNI）化学电离模式下进行分离和检测。该方法具有较高的灵敏度，SCCPs 和 MCCPs 的方法检测限（MDLs）分别低至 0.22μg/g 和 0.55μg/g。SCCPs 和 MCCPs 的方法总回收率分别为 104（±11）%和 108（±16）%。该方法成功应用于 NIST 标准参考物质（SRM 2585，房屋灰尘中的有机污染物）和加拿大房屋灰尘研究（CHDS）中收集的一部分房屋灰尘样品中 SCCPs 和 MCCPs 的分析。SRM 2585（n=12 个重复）中 SCCPs 和 MCCPs 的平均浓度分别为 7.58（±0.43）μg/g 和 16.4（±2.1）μg/g。比较了同一家庭使用两种不同方法收集的配对灰尘样品（FD 和 HD）中 CP 浓度：FD 是由技术人员收集的新鲜或“活性”灰尘，HD 是从家庭吸尘器中采集的样本。Spearman 秩分析显示，FD 和 HD 样品中 MCCPs 和 SCCPs 之间存在显著正相关（p<0.01）。每个房屋灰尘样品（n=48 个 HD 样品）中都检测到了 CP，SCCPs 和 MCCPs 的中位数（范围）浓度分别为 6.2（4.0-57）μg/g 和 19（5.9-901）μg/g。在这初步的 48 个 HD 样本中，CP 水平分布广泛，且 100%的检测频率表明加拿大家庭接触 CP 的情况存在很大差异。
