Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, China.
J Gene Med. 2019 Feb;21(2-3):e3071. doi: 10.1002/jgm.3071. Epub 2019 Feb 4.
The Maonan population is a relatively isolated minority in China. Little is known about endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations. The present study aimed to detect the association of several LIPG SNPs and environmental factors with serum lipid levels in the Chinese Maonan and Han populations.
In total, 773 subjects of Maonan ethnicity and 710 participants of Han ethnicity were randomly selected from our previous stratified randomized samples. Genotypes of the LIPG rs2156552, rs4939883 and rs7241918 SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism, and then confirmed by direct sequencing.
The allelic (rs2156552, rs4939883 and rs7241918) and genotypic (rs2156552 and rs4939883) frequencies were different between the two ethnic groups (p < 0.05-0.01). The minor allele carriers had lower apolipoprotein (Apo)A1/ApoB ratio (rs2156552 and rs7241918), high-density lipoprotein cholesterol (HDL-C) and apolipoprotein (Apo)A1 (rs2156552) levels and higher ApoB levels (rs4939883) in the Han population, and lower HDL-C (rs2156552, rs4939883 and rs7241918) levels in the Maonan minority than the minor allele non-carriers (p < 0.0167 after Bonferroni correction). Subgroup analyses according to sex showed that the minor allele carriers had a lower ApoA1/ApoB ratio (rs2156552 and rs7241918) and higher ApoB levels (rs7241918) in Han males, and lower ApoA1 and HDL-C levels in Maonan females than the minor allele non-carriers (p < 0.0167-0.001).
The present study demonstrates the association between the LIPG polymorphsims and serum lipid levels in the two ethnic groups. These associations might have an ethnic- and or/sex-specificity.
毛南族是中国相对孤立的少数民族之一。目前尚不清楚内皮脂肪酶基因（LIPG）单核苷酸多态性（SNPs）与中国人群的血清脂质水平之间的关系。本研究旨在检测 LIPG SNPs 与环境因素与中国毛南族和汉族人群血清脂质水平的相关性。
从我们之前的分层随机样本中，随机选择了 773 名毛南族和 710 名汉族参与者。通过聚合酶链反应-限制性片段长度多态性确定 LIPG rs2156552、rs4939883 和 rs7241918 SNPs 的基因型，然后通过直接测序进行确认。
两个民族之间的等位基因（rs2156552、rs4939883 和 rs7241918）和基因型（rs2156552 和 rs4939883）频率不同（p<0.05-0.01）。汉族人群中，携带 minor 等位基因的个体载脂蛋白（Apo）A1/ApoB 比值（rs2156552 和 rs7241918）、高密度脂蛋白胆固醇（HDL-C）和载脂蛋白（Apo）A1（rs2156552）水平较高，ApoB 水平（rs4939883）较高，而非 minor 等位基因携带者则较低（经 Bonferroni 校正后 p<0.0167）。按性别进行的亚组分析显示，汉族男性中，携带 minor 等位基因的个体载脂蛋白 A1/ApoB 比值（rs2156552 和 rs7241918）较低，ApoB 水平较高（rs7241918），而毛南族女性的载脂蛋白 A1 和 HDL-C 水平低于非 minor 等位基因携带者（经 Bonferroni 校正后 p<0.0167-0.001）。
本研究表明 LIPG 多态性与两个民族的血清脂质水平相关。这些关联可能具有种族和/或性别特异性。
