急性心肌梗死合并脑梗死与 MMP-2 和 MMP-9 表达水平的相关性。

Correlation between acute myocardial infarction complicated with cerebral infarction and expression levels of MMP-2 and MMP-9.

机构信息

Department of Rehabilitation Medicine, Zhejiang Provincial People's Hospital, and People's Hospital of Hangzhou Medical College; Hangzhou, China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Jan;23(1):297-302. doi: 10.26355/eurrev_201901_16776.

DOI:10.26355/eurrev_201901_16776

PMID:30657570

Abstract

OBJECTIVE

To investigate the correlation between the acute myocardial infarction (AMI) complicated with cerebral infarction (CI) and expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9.

PATIENTS AND METHODS

A total of 50 AMI patients treated in our hospital were enrolled, including 23 AMI patients complicated with CI in the AMI-CI group, and 27 patients with AMI alone in the AMI group. Venous blood was collected from each patient after admission. The serum levels of MMP-2 and MMP-9 were detected via enzyme-linked immunosorbent assay (ELISA), and their mRNA expressions were measured via quantitative Polymerase Chain Reaction (qPCR). The cerebral computed tomography (CT) scan was performed for calculating the CI size. The neurological function was evaluated using the neurological deficit score. The correlations between the levels of MMP-2 and MMP-9 with CI size and neurological deficit score were investigated using Pearson correlation analysis.

RESULTS

The expression levels of MMP-2 and MMP-9 in the AMI-CI group were significantly higher than those in the AMI group with significant differences (p<0.05). The mRNA expressions of MMP-2 and MMP-9 in AMI-CI group were also significantly higher than those in the AMI group, and the differences between the two groups were statistically significant (p<0.05). Larger CI size was observed in the AMI-CI group than that in the AMI group, showing a statistically significant difference between the two groups (p<0.05). The neurological deficit score in the AMI-CI group was significantly higher than that in the AMI group, with a statistically significant difference between the two groups (p<0.05). The expression levels of MMP-2 and MMP-9 were positively correlated with the CI size and neurological deficit score in AMI patients complicated with CI.

CONCLUSIONS

Disease severity of AMI complicated with CI is positively correlated with the expression levels of MMP-2 and MMP-9. Higher expression levels of MMP-2 and MMP-9 are expected to indicate a higher risk of AMI complicated with CI.

摘要

目的

探讨急性心肌梗死（AMI）并发脑梗死（CI）与基质金属蛋白酶-2（MMP-2）和 MMP-9 表达水平的相关性。

患者与方法

选取我院收治的 50 例 AMI 患者，其中 AMI 合并 CI 患者 23 例（AMI-CI 组），单纯 AMI 患者 27 例（AMI 组）。入院后采集每位患者的静脉血，采用酶联免疫吸附试验（ELISA）检测 MMP-2 和 MMP-9 的血清水平，采用实时荧光定量聚合酶链反应（qPCR）检测其 mRNA 表达水平。行脑 CT 扫描计算 CI 面积，采用神经功能缺损评分评估神经功能。采用 Pearson 相关分析探讨 MMP-2 和 MMP-9 水平与 CI 面积和神经功能缺损评分的相关性。

结果

AMI-CI 组 MMP-2 和 MMP-9 的表达水平明显高于 AMI 组，差异有统计学意义（p<0.05）。AMI-CI 组 MMP-2 和 MMP-9 的 mRNA 表达水平也明显高于 AMI 组，两组比较差异有统计学意义（p<0.05）。AMI-CI 组 CI 面积大于 AMI 组，两组比较差异有统计学意义（p<0.05）。AMI-CI 组神经功能缺损评分明显高于 AMI 组，两组比较差异有统计学意义（p<0.05）。AMI 合并 CI 患者 MMP-2 和 MMP-9 的表达水平与 CI 面积和神经功能缺损评分呈正相关。