Department of Biophysics, Bose Institute, Kolkata, 700054, India.
School of Nanoscience and Technology, IIT Kharagpur, Kharagpur, 721302, India.
Colloids Surf B Biointerfaces. 2019 Apr 1;176:360-370. doi: 10.1016/j.colsurfb.2019.01.020. Epub 2019 Jan 8.
Two-dimensional (2D) tungsten disulfide (WS) quantum dots offer numerous promising applications in materials and optoelectronic sciences. Additionally, the catalytic and photoluminescence properties of ultra-small WS nanoparticles are of potential interest in biomedical sciences. Addressing the use of WS in the context of infection, the present study describes the conjugation of two potent antimicrobial peptides with WS quantum dots, as well as the application of the resulting conjugates in antimicrobial therapy and bioimaging. In doing so, we determined the three-dimensional solution structure of the quantum dot-conjugated antimicrobial peptide by a series of high-resolution nuclear magnetic resonance (NMR) techniques, correlating this to the disruption of both model lipid and bacterial membranes, and to several key biological performances, including antimicrobial and anti-biofilm effects, as well as cell toxicity. The results demonstrate that particle conjugation enhances the antimicrobial and anti-biofilm potency of these peptides, effects inferred to be due to multi-dendate interactions for the conjugated peptides. As such, our study provides information on the mode-of-action of such conjugates, laying the foundation for their potential use in treatment and monitoring of infections.
二维（2D）二硫化钨（WS）量子点在材料和光电子科学领域具有许多有前途的应用。此外，超小 WS 纳米颗粒的催化和光致发光性能在生物医学科学中也具有潜在的应用价值。在感染方面应用 WS 的情况下，本研究描述了将两种有效的抗菌肽与 WS 量子点进行共轭，以及将所得共轭物应用于抗菌治疗和生物成像。为此，我们通过一系列高分辨率核磁共振（NMR）技术确定了量子点-共轭抗菌肽的三维溶液结构，将其与模型脂质和细菌膜的破坏以及包括抗菌和抗生物膜效应以及细胞毒性在内的几个关键生物学性能相关联。结果表明，颗粒共轭增强了这些肽的抗菌和抗生物膜效力，推断这种效果归因于共轭肽的多齿状相互作用。因此，我们的研究提供了有关此类共轭物作用模式的信息，为它们在治疗和监测感染中的潜在应用奠定了基础。
