CSIR- Central Scientific Instruments Organisation, Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Department of Biotechnology, UIET, Panjab University, Chandigarh, 160014, India.
Biosens Bioelectron. 2020 Mar 1;151:111979. doi: 10.1016/j.bios.2019.111979. Epub 2019 Dec 21.
A novel approach for the synthesis of biosurfactant stabilized/functionalized tungsten disulfide (WS-B) quantum dots (QDs) and its application for ferritin immunosensor is reported. These 2-D layered material derived quantum dots are synthesized via one-step liquid exfoliation method and biosurfactant was used as a functionalization and stability providing moiety. The biosurfactant provided a clean and green method for both the synthesis and in-situ functionalization of the QDs. Exhaustive characterization using analytical techniques was done to study various aspects of the synthesized quantum dots. The functionalized quantum dots (WS-B QDs) were further explored for their possible application as an electroactive platform. For this, the working area of commercially available screen-printed electrodes (SPE) was functionalized with these WS-B QDs to construct a sensor platform. This sensor platform was then used for fabrication of ferritin immunosensor, using ferritin specific antibodies. Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) techniques were used for electrochemical immunosensing of ferritin. Though, the achieved linear range for ferritin detection (10-1500 ng mL) is same with both the techniques but regression coefficient and limit of detection are better in differential pulse voltammetry. The limit of detection was found to be 3.800 ng mL in DPV and 6.048 ng mL in CV. The immunosensor is highly selective, reproducible and is stable for about 60 days.
本文报道了一种合成生物表面活性剂稳定/功能化二硫化钨（WS-B）量子点（QD）的新方法及其在铁蛋白免疫传感器中的应用。这些二维层状材料衍生的量子点是通过一步液相剥离法合成的，生物表面活性剂既作为功能化基团，又作为稳定性提供基团。生物表面活性剂为 QD 的合成和原位功能化提供了一种清洁和绿色的方法。采用各种分析技术进行了详尽的表征，以研究合成量子点的各个方面。进一步探索了功能化量子点（WS-B QD）作为电活性平台的可能应用。为此，用这些 WS-B QD 对市售的丝网印刷电极（SPE）的工作区域进行功能化，构建传感器平台。然后，使用铁蛋白特异性抗体在该传感器平台上制备铁蛋白免疫传感器。使用循环伏安法（CV）和差分脉冲伏安法（DPV）技术进行铁蛋白的电化学免疫传感。尽管两种技术的铁蛋白检测线性范围（10-1500 ng mL）相同，但差分脉冲伏安法的回归系数和检测限更好。在 DPV 中检测限为 3.800 ng mL，在 CV 中检测限为 6.048 ng mL。该免疫传感器具有高选择性、重现性，且在大约 60 天内稳定。
