Plant Physiology and Biochemistry Laboratory, Centre of Advanced Study, Department of Botany, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India.
Plant Molecular Cytogenetics Laboratory, Centre of Advanced Study, Department of Botany, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India.
Plant Physiol Biochem. 2019 Mar;136:76-91. doi: 10.1016/j.plaphy.2018.12.026. Epub 2018 Dec 28.
The activities of TCA cycle enzymes viz., pyruvate dehydrogenase, citrate synthase, isocitrate dehydrogenase, succinate dehydrogenase and malate dehydrogenase as well as levels of different organic acids viz., pyruvic acid, citric acid, succinic acid and malic acid were studied in two rice cultivars viz. cv. Nonabokra and cv. MTU 1010 differing in salt tolerance grown under 25, 50 and 100 mM NaCl salinity levels. A contrasting response to salt stress on enzyme activities of TCA cycle and accumulation of organic acid was observed between two cultivars during twenty-one days period of study. Salinity caused enhanced organic acid production and increase in all five enzyme activities in cv. Nonabokra whereas in cv. MTU 1010 decrease in both organic acid production and enzymes activities were noted. Joint application of exogenous silicon along with NaCl, altered the organic acids levels and activities of enzymes in both cultivars of rice seedlings conferring tolerance against salt induced stress. Rice cv. MTU 1010 showed better response to exogenous silicon on parameters tested compared to cv. Nonabokra.
研究了在 25、50 和 100 mM NaCl 盐度水平下生长的两种耐盐性不同的水稻品种（非那博克拉和 MTU1010）中 TCA 循环酶（如丙酮酸脱氢酶、柠檬酸合酶、异柠檬酸脱氢酶、琥珀酸脱氢酶和苹果酸脱氢酶）的活性以及不同有机酸（如丙酮酸、柠檬酸、琥珀酸和苹果酸）的水平。在 21 天的研究期间，两种品种对 TCA 循环酶的盐胁迫反应和有机酸的积累表现出相反的反应。盐胁迫导致非那博克拉中有机酸的产生和所有五种酶活性的增强，而在 MTU1010 中，有机酸的产生和酶活性都减少。外源硅与 NaCl 联合应用改变了两种水稻幼苗品种的有机酸水平和酶活性，赋予了它们对盐诱导胁迫的耐受性。与非那博克拉相比，MTU1010 对测试参数的外源硅表现出更好的响应。
