Kotebe Metropolitan University, P.O. Box 3268, Addis Ababa, Ethiopia.
Department of Medical Laboratory Science, CHS, AAU, Addis Ababa, Ethiopia.
BMC Complement Altern Med. 2019 Jan 18;19(1):24. doi: 10.1186/s12906-019-2429-4.
An alarm increase the rate of emerging and re-emerging of multidrug resistant bacteria have been caused great public health concern in the worldwide. They have been resisting for most or majority of currently available and affordable antibiotics and imposed socioeconomic catastrophe at global scale. As a result, there is utmost important to discover new or modify currently available antibiotics. The aim of this study was to evaluate combined antibacterial effect of essential oils obtained from Blepharis cuspidata, Boswellia ogadensis and Thymus schimper against multidrug resistance (MDR) Escherichia coli, Klebsiella pneumoniae and Methicillin resistant S. aureus.
Essential oil (EO) was extracted from the aerial part of B. cuspidata, B.ogadensis and T. schimper by steam distillation and stored in brown bottles at 4 °C. There were mixed in 1:1 ratio and adsorbed to disc and placed on MHA and measured their minimum inhibitory zone seeded with E. coli, K. pneumoniae and MRAS after 18-24 H. minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured by broth micro-dilution method. The interaction between EOs was determined by fractional inhibitory concentration index.
The antibacterial potential of mixed oil depends on the doses and type of the EOs and bacteria species. The combined EOs of B.cuspidata and T.schimperi had inhibition zone (39 mm), its MIC and MBC value was 0.39 μl/ml against MRSA. It had inhibition zone (28-35 mm), MIC value 0.39-6.25 μl/ml and MBC (0.78-12.5 μl/ml) against MDR E. coli and K. pneumoniae. Whereas, combined effects of B. cuspidata and B. ogadensis had MIC values ranges from 0.78-6.25 μl/ml for E.coli and K. pneumoniae and 1.56 μl/ml for MRSA. There was strong synergistic effect between the combination of B.cuspidata and T.schimperi. This study revealed that gram negative bacteria were slightly less susceptible than gram positive.
This in vitro study of combined EOs has significant antibacterial effect than using each of them and even it was more potent antibacterial effect on MDR as compare to modern antibiotics. Hence, it can be applied to a pharmaceutical composition as modulator or adjuvant or precursor for synthesis of new antibiotic in future activities.
警报增加了多药耐药菌的出现和再现率，这在全球范围内引起了极大的公共卫生关注。它们对大多数或大部分现有的、可负担得起的抗生素产生了耐药性，并在全球范围内造成了社会经济灾难。因此，发现新的或修改现有的抗生素是非常重要的。本研究的目的是评估从斑鸠菊、没药和麝香草中提取的精油对多药耐药（MDR）大肠杆菌、肺炎克雷伯菌和耐甲氧西林金黄色葡萄球菌的联合抗菌作用。
通过水蒸气蒸馏从斑鸠菊、没药和麝香草的地上部分提取精油，并储存在棕色瓶子中，储存在 4°C。将它们以 1:1 的比例混合并吸附到圆盘上，然后将其放置在 MHA 上，在接种了大肠杆菌、肺炎克雷伯菌和 MRSA 后 18-24 小时测量其最小抑菌区。通过肉汤微量稀释法测量最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。通过部分抑菌浓度指数（FICI）来确定精油之间的相互作用。
混合油的抗菌潜力取决于精油的剂量和类型以及细菌种类。B.cuspidata 和 T.schimperi 的混合精油对 MRSA 的抑菌圈（39mm）、MIC 值和 MBC 值分别为 0.39μl/ml。它对 MDR 大肠杆菌和肺炎克雷伯菌的抑菌圈（28-35mm）、MIC 值 0.39-6.25μl/ml 和 MBC（0.78-12.5μl/ml）。而 B.cuspidata 和 B.ogadensis 的联合作用对大肠杆菌和肺炎克雷伯菌的 MIC 值范围为 0.78-6.25μl/ml，对 MRSA 的 MIC 值为 1.56μl/ml。B.cuspidata 和 T.schimperi 的组合具有很强的协同作用。本研究表明，革兰氏阴性菌的敏感性略低于革兰氏阳性菌。
与单独使用每种精油相比，这种联合精油的体外研究具有显著的抗菌作用，甚至对 MDR 的抗菌作用比现代抗生素更强。因此，它可以应用于药物组合物中，作为未来活动中新抗生素的调节剂、佐剂或前体。
