Franz Alexander W E, Clem Rollie J, Passarelli A Lorena
Department of Veterinary Pathobiology, 303 Connaway Hall, College of Veterinary Medicine, University of Missouri, Columbia MO 65211, USA.
Molecular, Cellular, and Developmental Biology Program, Division of Biology, 116 Ackert Hall, Kansas State University, Manhattan, KS 66506, USA.
Curr Trop Med Rep. 2014 Mar 1;1(1):21-31. doi: 10.1007/s40475-013-0007-2.
is the principal vector of dengue virus (DENV) throughout the tropical world. This anthropophilic mosquito species needs to be persistently infected with DENV before it can transmit the virus through its saliva to a new vertebrate host. In the mosquito, DENV is confronted with several innate immune pathways, among which RNA interference is considered the most important. The genome project opened the doors for advanced molecular studies on pathogen-vector interactions including genetic manipulation of the vector for basic research and vector control purposes. Thus, has become the primary model for studying vector competence for arboviruses at the molecular level. Here, we present recent findings regarding DENV-mosquito interactions, emphasizing how innate immune responses modulate DENV infections in . We also describe the latest advancements in genetic manipulation of and discuss how this technology can be used to investigate vector transmission of DENV at the molecular level and to control transmission of the virus in the field.
是登革病毒（DENV）在整个热带地区的主要传播媒介。这种嗜人血的蚊种需要持续感染登革病毒，才能通过其唾液将病毒传播给新的脊椎动物宿主。在蚊子体内，登革病毒会面临多种先天免疫途径，其中RNA干扰被认为是最重要的。基因组计划为病原体与媒介相互作用的高级分子研究打开了大门，包括为基础研究和媒介控制目的对媒介进行基因操作。因此，已成为在分子水平上研究虫媒病毒媒介能力的主要模型。在这里，我们介绍了关于登革病毒与蚊子相互作用的最新发现，重点强调了先天免疫反应如何调节登革病毒在蚊子体内的感染。我们还描述了蚊子基因操作的最新进展，并讨论了如何利用这项技术在分子水平上研究登革病毒的媒介传播以及在野外控制病毒传播。
