Karimzadeh Zahra, Javanbakht Siamak, Namazi Hassan
Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran.
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Bioimpacts. 2019;9(1):5-13. doi: 10.15171/bi.2019.02. Epub 2018 Jul 18.
In recent years, more attention was dedicated to developing new methods for designing of drug delivery systems. The aim of present work is to improve the efficiency of the antibacterial drug delivery process, and to realize and to control accurately the release. First, graphene oxide (GO) was prepared according to the modified Hummers method then the GO was modified with carboxymethylcellulose (CMC) and Zn-based metal-organic framework (MOF-5) through the solvothermal technique. Performing the various analysis methods including scanning electron microscope (SEM), X-ray diffraction (XRD), EDX, Fourier transform infrared (FTIR) spectroscopy and Zeta potentials on the obtained bio-nanocomposite showed that the new modified GO has been prepared. With using common analysis methods the structure of synthesized materials was determined and confirmed and finally, their antibacterial behavior was examined based on the broth microdilution methods. Carboxymethylcellulose/MOF-5/GO bio-nanocomposite (CMC/MOF-5/GO) was successfully synthesized through the solvothermal technique. Tetracycline (TC) was encapsulated in the GO and CMC/MOF-5/GO. The drug release tests showed that the TC-loaded CMC/MOF5/GO has an effective protection against stomach pH. With controlling the TC release in the gastrointestinal tract conditions, the long-time stability of drug dosing was enhanced. Furthermore, antibacterial activity tests showed that the TC-loaded CMC/MOF-5/GO has an antibacterial activity to negatively charge bacteria in contrast to TC-loaded GO.
近年来，人们更加关注开发药物递送系统设计的新方法。本研究的目的是提高抗菌药物递送过程的效率，并准确实现和控制药物释放。首先，按照改良的Hummers方法制备氧化石墨烯（GO），然后通过溶剂热技术用羧甲基纤维素（CMC）和锌基金属有机框架（MOF-5）对GO进行改性。对所得生物纳米复合材料进行扫描电子显微镜（SEM）、X射线衍射（XRD）、能谱分析（EDX）、傅里叶变换红外（FTIR）光谱和Zeta电位等各种分析方法，结果表明已制备出新型改性GO。通过常用分析方法确定并证实了合成材料的结构，最后，基于肉汤微量稀释法检测了它们的抗菌性能。通过溶剂热技术成功合成了羧甲基纤维素/MOF-5/GO生物纳米复合材料（CMC/MOF-5/GO）。将四环素（TC）包封在GO和CMC/MOF-5/GO中。药物释放试验表明，负载TC的CMC/MOF5/GO对胃内pH具有有效的保护作用。通过控制胃肠道条件下TC的释放，增强了药物给药的长期稳定性。此外，抗菌活性试验表明，与负载TC的GO相比，负载TC的CMC/MOF-5/GO对带负电荷的细菌具有抗菌活性。
