Por Yong-Chen, Yeow Vincent Kok-Leng, Louri Nayef, Lim Tony Kiat-Hon, Kee Irene, Song In-Chin
Department of Plastic Surgery, Kandang Kerbau Women's and Children's Hospital, Singapore.
J Plast Reconstr Aesthet Surg. 2009 Aug;62(8):1030-4. doi: 10.1016/j.bjps.2008.01.013. Epub 2008 Jun 11.
Free fat grafts have an unpredictable survival rate, which may be dependent on host bed vascularity. Therefore, the authors hypothesized that the presence of growth factors in platelet-rich plasma (PRP), may enhance free fat graft survival.
Free fat grafts and autologous PRP were harvested from a healthy female and processed using the Coleman technique and the Medtronic Magellan system respectively. The experiment comprised two groups of 12 nude mice each with injection of free fat grafts into the scalp. The experimental group comprised the combination of 0.8 ml of free fat graft and 0.2 ml of PRP. The control group comprised the combination of 0.8 ml of free fat graft and 0.2 ml of normal saline. The mice were euthanized after 16 weeks and the fat grafts explanted and measured for weight and volume. Histology was performed with Oil Red O stain. Statistical analysis of the weight and volume in between groups was performed using the independent samples T-test (SPSS v11). The Mann-Whitney test was used to compare the ranking of six histological parameters between the two groups.
The mean weight and volume for the experimental arm were 0.503 g and 0.545 ml respectively. The mean weight and volume for the control arm were 0.500 g and 0.541 ml respectively. The weight, volume and histological parameters between the two groups were not statistically significant. A mouse from each group died of unknown causes.
PRP did not enhance free fat graft survival in the nude mouse.
游离脂肪移植的成活率难以预测，这可能取决于受区血管情况。因此，作者推测富含血小板血浆（PRP）中的生长因子可能会提高游离脂肪移植的成活率。
从一名健康女性身上获取游离脂肪移植组织和自体PRP，分别采用科尔曼技术和美敦力麦哲伦系统进行处理。实验包括两组，每组12只裸鼠，将游离脂肪移植组织注射到头皮中。实验组为0.8毫升游离脂肪移植组织与0.2毫升PRP的组合。对照组为0.8毫升游离脂肪移植组织与0.2毫升生理盐水的组合。16周后对小鼠实施安乐死，取出脂肪移植组织并测量其重量和体积。采用油红O染色进行组织学检查。使用独立样本t检验（SPSS v11）对两组之间的重量和体积进行统计分析。采用曼-惠特尼检验比较两组之间六个组织学参数的排名。
实验组的平均重量和体积分别为0.503克和0.545毫升。对照组的平均重量和体积分别为0.500克和0.541毫升。两组之间的重量、体积和组织学参数无统计学意义。每组各有一只小鼠死因不明。
在裸鼠中，PRP并未提高游离脂肪移植的成活率。
