Selux Diagnostics, Inc., 56 Roland Street, Suite 206, Charlestown, MA, 02129, USA.
Massachusetts General Hospital, 55 Fruit St, Boston, MA, 02114, USA.
Sci Rep. 2019 Jan 18;9(1):237. doi: 10.1038/s41598-018-35916-0.
Rapid delivery of proper antibiotic therapies to infectious disease patients is essential for improving patient outcomes, decreasing hospital lengths-of-stay, and combating the antibiotic resistance epidemic. Antibiotic stewardship programs are designed to address these issues by coordinating hospital efforts to rapidly deliver the most effective antibiotics for each patient, which requires bacterial identification and antimicrobial susceptibility testing (AST). Despite the clinical need for fast susceptibility testing over a wide range of antibiotics, conventional phenotypic AST requires overnight incubations, and new rapid phenotypic AST platforms restrict the number of antibiotics tested for each patient. Here, we introduce a novel approach to AST based on signal amplification of bacterial surfaces that enables phenotypic AST within 5 hours for non-fastidious bacteria. By binding bacterial surfaces, this novel method allows more accurate measurements of bacterial replication in instances where organisms filament or swell in response to antibiotic exposure. Further, as an endpoint assay performed on standard microplates, this method should enable parallel testing of more antibiotics than is currently possible with available automated systems. This technology has the potential to revolutionize clinical practice by providing rapid and accurate phenotypic AST data for virtually all available antibiotics in a single test.
迅速为传染病患者提供适当的抗生素治疗对于改善患者预后、缩短住院时间和对抗抗生素耐药性的流行至关重要。抗生素管理计划旨在通过协调医院的努力，为每个患者快速提供最有效的抗生素来解决这些问题，这需要进行细菌鉴定和抗菌药物敏感性测试（AST）。尽管临床上需要对广泛的抗生素进行快速药敏测试，但传统的表型 AST 需要过夜孵育，而新的快速表型 AST 平台限制了每个患者的测试抗生素数量。在这里，我们介绍了一种基于细菌表面信号放大的新型 AST 方法，该方法可在 5 小时内对非苛养菌进行表型 AST。通过结合细菌表面，这种新方法可以在生物体因抗生素暴露而丝状或肿胀的情况下更准确地测量细菌的复制。此外，作为在标准微孔板上进行的终点测定，该方法应该能够比目前可用的自动化系统同时测试更多的抗生素。这项技术有可能通过在单次测试中为几乎所有可用的抗生素提供快速准确的表型 AST 数据，从而彻底改变临床实践。
