Department of Biochemistry, Covenant University, Ogun State 112233, Nigeria.
Department of Biochemistry, Afe Babalola University, Ado-Ekiti 360001, Nigeria.
J Integr Med. 2019 Mar;17(2):125-131. doi: 10.1016/j.joim.2019.01.002. Epub 2019 Jan 5.
This study investigated the ameliorative potential of Zingiber officinale Roscoe extract against lead-induced brain damage in rats.
Thirty male rats were divided into 5 groups of 6 rats each. Lead-acetate toxicity was induced by intraperitoneal injection (10 mg/kg body weight (b.w.)) in Groups B-E. Group A (control) and Group B (lead-acetate) were left untreated; vitamin C (200 mg/kg b.w.) was administered to Group C; ethyl acetate fraction from Z. officinale extract (200 and 100 mg/kg b.w.) was administered to Group D and E by oral gavage once daily for 7 days. Changes in the content of some key marker enzymes such as acetylcholinesterase (AChE), butyrylcholinesterase (BChE), monoamine oxidase (MAO), epinephrine, dopamine, Na/K-ATPase, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as malonaldehyde (MDA) levels were determined in serum.
Exposure to lead acetate resulted in a significant decrease (P < 0.05) in the activities of BChE, AChE, Na/K-ATPase, SOD, CAT and GPx with a corresponding increase in the levels of MDA, xanthine oxidase, epinephrine, dopamine and MAO relative to the control group. Levels of all disrupted parameters were alleviated by co-administration of Z. officinale fraction and by the standard drug, vitamin C.
These results suggest that ethyl acetate fraction of Z. officinale extract attenuates lead-induced brain damage and might have therapeutic potential as a supplement that can be applied in lead poisoning.
本研究旨在探讨姜黄提取物对大鼠铅诱导脑损伤的改善作用。
将 30 只雄性大鼠分为 5 组，每组 6 只。采用腹腔注射（10mg/kg 体重）醋酸铅诱导大鼠铅中毒，B-E 组。A 组（对照组）和 B 组（醋酸铅组）未予处理；C 组给予维生素 C（200mg/kg 体重）；D、E 组每日灌胃给予姜黄提取物乙酸乙酯部位（200、100mg/kg 体重），连续 7 天。测定血清中一些关键标志物酶（如乙酰胆碱酯酶、丁酰胆碱酯酶、单胺氧化酶、肾上腺素、多巴胺、Na/K-ATP 酶、过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）的含量和丙二醛（MDA）水平。
醋酸铅暴露导致 BChE、AChE、Na/K-ATP 酶、SOD、CAT 和 GPx 活性显著降低（P<0.05），MDA、黄嘌呤氧化酶、肾上腺素、多巴胺和 MAO 水平相应升高。与对照组相比，姜黄提取物乙酸乙酯部位和标准药物维生素 C 均可减轻所有这些参数的紊乱。
这些结果表明，姜黄提取物乙酸乙酯部位可减轻铅诱导的脑损伤，作为一种补充剂，可能具有治疗铅中毒的潜力。
