School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.
School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Centro de Salud las Palmas, Red de Salud Leoncio Prado, Tingo María, Peru.
Int J Infect Dis. 2019 Apr;81:31-37. doi: 10.1016/j.ijid.2019.01.022. Epub 2019 Jan 17.
Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus.
A total of 268 serum samples from patients in Huánuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016.
DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods CONCLUSIONS: In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru.
万卡约是秘鲁中东部的一个地区，其地理环境包括高森林和低丛林，以及构成安第斯山脉山谷的山脉。由于存在许多有利于病毒传播的有利条件，因此该地区被认为是登革热的地方病。
2015 年 12 月至 2016 年 3 月，对秘鲁万卡约地区患有急性发热病的 268 例血清样本进行了登革热病毒（DENV）的 RT-PCR 和 NS1、IgM 和 IgG ELISA 检测。
通过 RT-PCR 在 25％的样本中检测到 DENV，通过 NS1 抗原 ELISA 在 19％的样本中检测到 DENV，通过 IgM ELISA 在 10.5％的样本中检测到 DENV。通过 ELISA 在 15.7％的样本中检测到 DENV IgG。所有组中与发热相关的最常见症状是头痛，肌痛和关节痛，四种检测方法之间无显着差异。
在这项研究中，使用标准实验室方法在多达 25％的样本中鉴定出 DENV。此外，还建立了 NS1、IgM 和 IgG 确定的血清学检测与阳性结果频率之间的相关性。迫切需要进行即时检测以加强秘鲁的流行病学监测。
