Laboratory of Phytochemistry, Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaounde, Cameroon.
Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM), University of Liege, Liege, Belgium.
Nat Prod Res. 2020 Nov;34(22):3182-3188. doi: 10.1080/14786419.2018.1553879. Epub 2019 Jan 19.
One new limonoid, trigilgianin (), one new phenyl alkene, epoxy gilgialkene (), together with five known compounds: scopoletin (), sitosteryl-6'--undecanoate--D-glucoside (), sitosteryl---D-glucopyranoside (), cinchonain A () and cinchonain B () were isolated from the stem bark of Harms. (Meliaceae). All compounds were isolated for the first time from this species. The structures were elucidated on the basis of spectral studies and by comparison of these data with those from the literature. Compounds , , , and were tested for antileishmanial activity against visceral leishmaniasis parasite and cytotoxicity against macrophage RAW 264.7 cell line. Compounds and showed the highest antileishmanial activity (IC values of 6.044 and 6.804 µg/mL, respectively) with low cytotoxicity (CC values of >200 and 47.47 µg/mL, respectively), while compound was moderately active on promastigotes (IC 56.81 µg/mL).
从 Harms 的茎皮中分离得到一种新的柠檬苦素 trigilgianin ()，一种新的苯乙烯 epoxy gilgialkene ()，以及五种已知化合物：东莨菪苷 ()、豆甾醇-6'-十一烷酸酯-D-葡萄糖苷 ()、豆甾醇---D-吡喃葡萄糖苷 ()、辛可宁 A ()和辛可宁 B ()。所有化合物均首次从该物种中分离得到。基于光谱研究并通过将这些数据与文献中的数据进行比较，确定了这些化合物的结构。测试了化合物 、 、 、 和 对内脏利什曼原虫寄生虫的抗利什曼原虫活性和对巨噬细胞 RAW 264.7 细胞系的细胞毒性。化合物 和 表现出最高的抗利什曼原虫活性（IC 值分别为 6.044 和 6.804 μg/mL，具有低细胞毒性（CC 值>200 和 47.47 μg/mL），而化合物 对前鞭毛体具有中等活性（IC 56.81 μg/mL）。
