Bioassay-guided isolation of vilasinin-type limonoids and phenyl alkene from the leaves of and their antiplasmodial activities.

Bioassay-guided fractionation of the CHCl-MeOH (1:1) leaves extract of , yielded two new vilasinin-type limonoids named gilgianin A () and gilgianin B (), one new phenyl alkene derivative designated as gilgialkene A (), along with six known compounds: rubescin H (), TS3 (), trichirubine A (), sitosteryl-6'--undecanoate--D-glucoside (), scopoletin (), and octadecane-2-one (). Their structures were elucidated based on spectroscopic analysis and comparison with literature data. Compounds and exhibited the highest antiplasmodial activity with IC values of 1.14 and 1.32 μM respectively. Moreover, compound was very cytotoxic with CC value of 0.88 µM, compared to compound , which was not cytotoxic (CC > 10 µg/mL). Compounds IC = 9.84 µM), (IC = 11.04 µM) and (IC = 10.71 µM) presented good antiplasmodial activity while also exhibiting significant cytotoxicity, with CC values ranging from of 14.45 to 29.7 µM.[Formula: see text].