Melong Raduis, Tamokoue Kengne Pierre Carolle, Dzoyem Jean Paul, Fusi Angelbert Awantu, Allemann Eric, Delie Florence, Bochet Christian G, Beifuss Uwe, Kapche Gilbert Deccaux Wabo Fotso
Department of Fundamental Science, Higher Technical Teachers' Training College, University of Bamenda, Bambili, Bamenda, North West Region, Cameroon.
Department of Chemistry, Faculty of science and Medicine, University of Fribourg, Fribourg, Switzerland.
Nat Prod Res. 2022 Jun;36(11):2783-2790. doi: 10.1080/14786419.2021.1927024. Epub 2021 May 17.
The phytochemical study of the CHCl- MeOH (1:1, ) extract of the stem bark of DC (Meliaceae) led to the isolation and characterisation of a new natural limonoid 7-obacunol (), along with seven known compounds. Their structures were elucidated by spectroscopic means, including 1 D and 2 D NMR, HRESI-MS and by comparison with published data. The cytotoxicity of compounds was assessed by the WST-1 assay on human lung adenocarcinoma A549 and Raw 264.7 mouse macrophage cell lines. Results suggested that obacunone () exhibited the most potent cytotoxic effect against A549 and Raw 264.7 cells with respective IC values of 25.24 µM and 29.14 µM, while the new natural limonoid 7-obacunol () exhibited 32.75 µM and 39.19 µM, respectively on both cell lines. Therefore, limonoid derivatives might be promising sources of natural bioactive metabolites against cancer.
对楝科植物印楝（Azadirachta indica A. Juss.）茎皮的氯仿 - 甲醇（1:1）提取物进行植物化学研究，从中分离并鉴定出一种新的天然柠檬苦素7 - 奥巴库醇（7 - obacunol），以及七种已知化合物。通过光谱手段，包括一维和二维核磁共振、高分辨电喷雾电离质谱（HRESI - MS）并与已发表数据进行比较，阐明了它们的结构。采用WST - 1法在人肺腺癌A549细胞系和Raw 264.7小鼠巨噬细胞系上评估了这些化合物的细胞毒性。结果表明，奥巴库酮（obacunone）对A549和Raw 264.7细胞表现出最强的细胞毒性作用，其IC值分别为25.24 μM和29.14 μM，而新的天然柠檬苦素7 - 奥巴库醇（7 - obacunol）在这两种细胞系上的IC值分别为32.75 μM和39.19 μM。因此，柠檬苦素衍生物可能是有前景的抗癌天然生物活性代谢物来源。
