Laboratory of Bioflavors, Department of Food Science, School of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil.
Laboratory of Food Biotechnology, Food Engineering, Institute of Science and Technology, UFVJM, Diamantina, Minas Gerais, Brazil.
Biotechnol Adv. 2019 Mar-Apr;37(2):319-339. doi: 10.1016/j.biotechadv.2019.01.007. Epub 2019 Jan 18.
Nowadays, food, cosmetic, environmental and pharmaceutical fields are searching for alternative processes to obtain their major products in a more sustainable way. This fact is related to the increasing demand from the consumer market for natural products to substitute synthetic additives. Industrial biotechnology appears as a promising area for this purpose; however, the success of its application is highly dependent of the availability of a suitable microorganism. To overcome this drawback, the isolation of microorganisms from diverse sources, including fermented food, adverse environments, contaminated samples or agro-industrial wastes is an important approach that can provide a more adaptable strain able to be used as biocatalyst and that exhibit resistance to industrial conditions and high yields/productivities in biotechnological production of natural compounds. The aim of this review is to provide a solid set of information on the state of the art of isolation and screening studies for obtaining novel biocatalysts able to produce natural compounds, focusing in aromas, biosurfactants, polysaccharides and microbial oils.
如今,食品、化妆品、环境和制药领域都在寻找替代工艺,以更可持续的方式获得主要产品。这一事实与消费者市场对天然产品替代合成添加剂的需求不断增加有关。工业生物技术似乎是实现这一目标的一个有前途的领域;然而,其应用的成功在很大程度上取决于是否有合适的微生物。为了克服这一缺点,从各种来源(包括发酵食品、恶劣环境、污染样品或农业工业废物)中分离微生物是一种重要的方法,它可以提供更适应的菌株,能够用作生物催化剂,并表现出对工业条件的抗性,以及在天然化合物的生物技术生产中具有高产量/生产力。本文综述的目的是提供一套关于分离和筛选研究的最新信息,以获得能够生产天然化合物的新型生物催化剂,重点是香气、生物表面活性剂、多糖和微生物油。