College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
J Photochem Photobiol B. 2019 Mar;192:26-33. doi: 10.1016/j.jphotobiol.2018.12.009. Epub 2018 Dec 19.
The objective of this study was to investigate the effect of Mycosporine-like amino acids (MAAs) extracted from Porphyra tenera skin against UV irradiation-induced photoaging using an ICR mouse model of skin photoaging and to explore the curative effects of the compounds in MAAs. The skin damage and collagenous tissue impairments induced by ultraviolet radiation were observed by histopathological analysis, and the effects of MAAs on protecting against skin damage and maintaining an intact structure of collagenous tissue were studied. The expression of NF-κB and the MAPK signaling pathway and nuclear transcription factors MMP-1, MMP-3 and TNF-α was analyzed used quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and ELISA assays. The results showed that the MAAs extracted from Porphyra tenera contained Porphyra-334 and shinorine, which could prevent skin photoaging induced by ultraviolet irradiation and reduce the damage to collagen and elastin. Meanwhile, MAAs significantly inhibited the decrease in hydroxyproline and collagen content and protected against pathological damage to collagen fibers in photoaging skin. MAAs resulted in a reduction in the expression of interstitial collagenase (MMP-1), matrix metalloproteinases (MMP-3) and tumor necrosis factor-α (TNF-α) and a reduction in the content of tissue matrix metalloproteinase (MMPs). Furthermore, MAAs may promote procollagen synthesis by downregulating the expression of TNF-α and downregulating the levels of MMPs, which demonstrates that MAAs are involved in matrix collagen synthesis by activating the NF-kB pathway in photoaging skin. Based on these results, we concluded that MAAs protect skin from UV irradiation-induced photodamage, and therefore, MAAs may be a potentially effective agent for the prevention of photoaging.
本研究旨在通过建立皮肤光老化 ICR 小鼠模型，研究从紫菜（Porphyra tenera）皮中提取的菌多酚（MAAs）对紫外线照射诱导的光老化的影响，并探讨 MAAs 化合物的治疗效果。通过组织病理学分析观察紫外线辐射引起的皮肤损伤和胶原组织损伤，并研究 MAAs 对保护皮肤免受损伤和维持胶原组织完整结构的作用。采用定量实时逆转录聚合酶链反应（qRT-PCR）和酶联免疫吸附测定（ELISA）分析 MAAs 对核转录因子 NF-κB 和 MAPK 信号通路以及基质金属蛋白酶（MMPs）-1、MMP-3 和肿瘤坏死因子-α（TNF-α）表达的影响。结果表明，紫菜中提取的 MAAs 含有 Porphyra-334 和 shinorine，可预防紫外线照射引起的皮肤光老化，减少对胶原和弹性蛋白的损伤。同时，MAAs 可显著抑制羟脯氨酸和胶原含量的减少，防止光老化皮肤胶原纤维的病理性损伤。MAAs 导致间质胶原酶（MMP-1）、基质金属蛋白酶（MMP-3）和肿瘤坏死因子-α（TNF-α）表达减少，组织基质金属蛋白酶（MMPs）含量减少。此外，MAAs 可能通过下调 TNF-α 的表达和下调 MMPs 水平来促进前胶原合成，这表明 MAAs 通过激活光老化皮肤中的 NF-kB 通路参与基质胶原合成。基于这些结果，我们得出结论，MAAs 可保护皮肤免受紫外线照射引起的光损伤，因此，MAAs 可能是预防光老化的一种潜在有效药物。
